Product: APC/Fire™ 750 anti-mouse CD8a |
Catalog No.: 100765 |
Mahesh Raundhal, Postdoctoral Associate University of Pittsburgh |
Excellent Stain - Does not Bleed into APC! (APC/Fire™ 750 anti-mouse CD8a) | |||
Overall: | Product Quality: | Ease of Use: | |
Works great, no compensation required with APC! |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | Single cell suspension of mouse lungs |
Brief Protocol: | Lungs from anaesthetised naive mice were excised and digested in sterile Collagenase/DNase solution. After extensive washing, cells were resuspended in flow cytometry staining buffer and stained with PE-CF594 anti-CD3 and APC/Fire 750 anti-CD8a. |
Results Summary: | ~ 30% of total CD3+ cells were found to be positive for CD8. |
Additional Notes: |
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