Application: |
Flow cytometry - Surface staining |
Cells used: |
Mouse peripheral blood |
Brief Protocol: |
100ul peripheral blood was ACK lysed, washed twice with FACS buffer (PBS w/2% FBS and 0.09% sodium azide), and stained for 20 minutes at room temperature with a 1:200 dilution of anti-CD45 APC. Cells were washed twice and analyzed on a BD LSR II. |
Results Summary: |
CD45 staining was excellent. |
Additional Notes: |
The mice in this experiment were transplanted with bone marrow from GFP+ donor mice, therefore a majority of WBCs are GFP+. |
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