Product: PE anti-human CX3CR1 |
Catalog No.: 355703 |
Katherine Santostefano, Research Coordinator University of Florida |
CX3CR1 Expression in Primary Human Monocytes | |||
Overall: | Product Quality: | Ease of Use: | |
Worked well to detect receptor expression. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Primary human monocytes |
Brief Protocol: | Cells were fixed in 4% paraformaldehyde for 15 min Washed and stained with 5 ul/million cells with isotype control (catalog number 400113) or CX3CR1-PE antibody for 30 min on ice After washing, cells were resuspended in FACS buffer (PBS with 2% FCS)and analyzed on a BD Accuri C6 flow cytometer |
Results Summary: | CX3CR1 expression was easy to visualize compared to the isotype control. |
Additional Notes: |
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