Product: Brilliant Violet 605™ anti-human CD45RO |
Catalog No.: 304237 |
Max Hübner, Researcher LMU |
CD45RO for Labeling of Human PBMCs (Brilliant Violet 605™) | |||
Overall: | Product Quality: | Ease of Use: | |
This antibody was included in a panel for identifying effector memory T cell subpopulations in human primary PBMCs. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | Human PBMCs |
Brief Protocol: | Fc Block 10 mins room temperature with Tru Stain Fc Blocking Solution. 5µl of antibody on 2 millions cells, 30 mins on ice in the dark (antibody), wash three times afterwards with 2ml of FACS buffer (HBSS with 1%BSA and 0,01% Sodium Azide). |
Results Summary: | This antibody works with the dilutions mentioned above. |
Additional Notes: | Be sure to Fc-Block samples (10 Minutes, room temperature), wash three times after staining. |
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