Product: Purified anti-human CD38 |
Catalog No.: 303502 |
Katherine Nicholas, PhD Candidate Vanderbilt University |
Purified anti-human CD38 for CyTOF® | |||
Overall: | Product Quality: | Ease of Use: | |
Purified CD38 was conjugated to 148Nd and worked well in a mass cytometry panel. |
Experimental Design |
Application: | Mass cytometry |
Cells used: | Human PBMC |
Brief Protocol: | We conjugated purified CD38 to 148Nd using a MaxPar® Antibody Labeling Kit (we followed the kit instructions exactly). We then used our conjugated CD38-148Nd in a mass cytometry panel to characterize human PBMCs. We looked at CD38 expression with and without SEB stimulation and found that on the CyTOF® we detected comparable frequencies of CD38+ T and B cells as we did with fluorescence cytometry (R=0.87, p=0.0002). |
Results Summary: | We conjugated CD38 to 148Nd and used it in a mass cytometry panel. It detected the same frequency of CD40L+ singlets as fluorescence cytometry did (using CD40L-PE) in 6 subjects (R=0.87, p=0.0002). |
Additional Notes: |
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