Product: Brilliant Violet 605™ anti-human CD38 |
Catalog No.: 303532 |
Anne Rissiek, Dr. Universitätsklinikum Hamburg-Eppendorf (UKE) |
Anti-human CD38 Brilliant Violet 605™ | |||
Overall: | Product Quality: | Ease of Use: | |
Phenotyping CD38 expression by lymphocytes. |
Experimental Design |
Application: | FACS, staining human whole blood |
Cells used: | Human EDTA blood |
Brief Protocol: | 1. 100 µl EDTA blood plus 50 µl antibody cocktail (in FACS Buffer), incubate for 30 min at RT. 2. Lyse red good cells by adding 1 ml Lysis Buffer, incubate for 10 min at RT, wash 2x with PBS, 400 g, 5 min, trash supernatant. 3. Resuspend cells in 200-300 µl FACS Buffer, FACS analysis The CD38 antibody was titrated and applied at a final dilution of 1:200. |
Results Summary: | The expression of CD38 can be analyzed within lymphocyte subsets. |
Additional Notes: | Gating strategy: Lymphocytes --> Single cells --> not T (CD3+) an not B (CD19+) --> CD56. |
|
|
See all Reviews |
Follow Us