Product: Purified anti-Tubulin β 3 (TUBB3)

Catalog No.: 801202

Brian Lin, Grad Student Tufts University

mTuj1+ immature neurons in uninjured mouse olfactory epithelium
Overall:	Product Quality:	Ease of Use:
Excellent staining of immature neurons of the olfactory epithelium using mTuj1 antibody

Experimental Design

Application:	Fluorescent IHC
Cells used:	9 um cryostat cut fixed frozen sections
Brief Protocol:	Tissue was rehydrated and then steamed in citrate buffer for 10 minutes to allow for mouse on mouse staining. Blocked with donkey serum block, non-fat dry milk, and BSA. 1 hour RT incubation with primary mTuj1 at 1:400 dilution. 1 hour RT incubation with 1:150 Alexa Fluor 647 donkey anti-mouse antibody.
Results Summary:	Excellent staining of immature neurons, both cell body, axons in the nerve bundle, and apical dendritic processes.
Additional Notes:

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Publication Status This data has not been published.

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