Product: Purified anti-mouse CD8a

Catalog No.: 100702

Robert Yuan, Graduate Student Stanford

CD8+ T-cells are Clearly Stained with the Antibody
Overall:	Product Quality:	Ease of Use:
The antibody stains CD8 T-cells in secondary lymphoid structures in the colon.

Experimental Design

Application:	Immunofluorescence
Cells used:	Pre-fixed frozen section of a mouse colon
Brief Protocol:	The colon was harvested from a mouse and fixed in paraformaldehyde prior to embedding in cutting medium. A section was then obtained and blocked with 5% goat serum then stained with the anti-CD8a antibody overnight. An Alexa Fluor® 488-conjugated secondary antibody was used to visualize the stain.
Results Summary:	The antibody stains CD8 T-cells in the colon well.
Additional Notes:

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Publication Status This data has not been published.

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