||Detection of IL-1ÃŽÂ² release from microglia
||We generated mixed glial cell cultures from neonatal mice (p0-2) in 24-well plates and stimulated the cells for 2h with LPS to induce production of pro-IL-1ÃŽÂ² in microglia. We subsequently stimulated cells with 1.5mM ATP to induce P2X7-mediated inflammasome formation and cleavage of pro-IL-1ÃŽÂ² and release of IL-1ÃŽÂ² into the supernatant. Latter was quantified using Mouse IL-1ÃŽÂ² ELISA MAX™ Deluxe.
||Supernatants contained 100-300pg/ml mouse IL-1ÃŽÂ², depending on the number of microglia in the mixed glial cell culture.