Product: APC anti-mouse/human CD11b

Catalog No.: 101211

Dinesh K Ahirwar, Post Doctoral Researcher The Ohio State University

CD11b Antibody (101211) Successfully Detect Myeloid Cells
Overall:	Product Quality:	Ease of Use:
The CD11b antibody successfully detects CD11b positive myeloid/macrophage cells using flow cytometry.

Experimental Design

Application:	Flow cytometry
Cells used:	Primary cells isolated from MMTV-PyMT, orthotopic, Xenograft mouse models
Brief Protocol:	Pulverize the tumors to obtain single cell suspension using Collagenase IV. Wash the cells with DMEM complete media followed by PBS. Incubate the cells in 5% serum in TBS buffer for 30 min. on ice. Remove blocking buffer and add anti-CD11b antibody. The antibody is diluted to 1:100 in blocking buffer. Incubate the cells for 30 min. on ice. Wash the cells 3 times with PBS. Fix the cells with 2% paraformaldehyde in PBS. Analyze the cells with FACS.
Results Summary:	The anti-CD11b antibody successfully detects CD11b+ myeloid/macrophage cells in FACS analysis.
Additional Notes:

Data Image

Publication Status This data has been published. Pubmed

See all Reviews