Product: APC/Cyanine7 anti-mouse/human CD11b

Catalog No.: 101226

Vikram Mavinkurve, Mr. TTUHSC

CD11b APC/Cyanine7 anti-mouse Antibody for the analysis of Macs, Dendritic Cells and MDSCs
Overall:	Product Quality:	Ease of Use:
CD11b APC/Cyanine7 anti-mouse works quite well as a marker for myeloid cells and has been used extensively in our lab

Experimental Design

Application:	Flow Cytometry
Cells used:	Splenocytes, Tumor cells, Liver cell suspensions, Bone marrow derived macrophages
Brief Protocol:	Cells are washed with FACS buffer (1%FBS in 1X PBS). CD11b APC/Cyanine7 anti-mouse antibody is used as a surface stain at a concentration of 1:200 along with Fc block(anti-CD16/32) for 45 mins at 4C in FACS buffer. Cells are then fixed using Foxp3 Fix/Perm buffer overnight, then washed with FACS and run in the flow cytometer.
Results Summary:	Good separation of CD11b positive and negative populations were noticed, that helped in the analysis of myeloid cells
Additional Notes:	Antibody is not very bright, however, since the receptor CD11b is quite strongly expressed in these cells, the antibody can be used with great success and populations are easily identified. Has no problems with compensation even when used along with fluorophores like APC.

Data Image

Publication Status This data has been published. Pubmed

Technical Service Notes

Please note fixation is only recommended for 15-20 min. Extended fixation times can decrease antibody fluorescence.

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