Application: |
Flow Cytometry |
Cells used: |
Splenocytes, Tumor cells, Liver cell suspensions, Bone marrow derived macrophages |
Brief Protocol: |
Cells are washed with FACS buffer (1%FBS in 1X PBS). CD11b APC/Cyanine7 anti-mouse antibody is used as a surface stain at a concentration of 1:200 along with Fc block(anti-CD16/32) for 45 mins at 4C in FACS buffer. Cells are then fixed using Foxp3 Fix/Perm buffer overnight, then washed with FACS and run in the flow cytometer. |
Results Summary: |
Good separation of CD11b positive and negative populations were noticed, that helped in the analysis of myeloid cells |
Additional Notes: |
Antibody is not very bright, however, since the receptor CD11b is quite strongly expressed in these cells, the antibody can be used with great success and populations are easily identified. Has no problems with compensation even when used along with fluorophores like APC. |
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