Product: PE anti-mouse CD19

Catalog No.: 115508

J Lykken, Dr. Duke University

Clearly Labels CD19+ Peripheral Blood Mononuclear Cells
Overall:	Product Quality:	Ease of Use:
Clearly and consistently labels CD19+ peripheral blood mononuclear cells.

Experimental Design

Application:	Flow Cytometry
Cells used:	Peripheral Blood Mononuclear Cells
Brief Protocol:	Isolate peripheral blood from mice. Wash cells once in FACS buffer (PBS plus 1% BSA or serum). Block Fc receptors and stain for viability in PBS for 20 minutes on ice. Wash cells once in FACS buffer (PBS plus 1% BSA or serum). Stain with isotype control or CD19 antibody (0.4 ug/mL in FACS buffer, 1:500 dilution, 100 uL per million cells) for 20 minutes on ice. Wash cells once in FACS buffer. Lyse red blood cells. Resuspend cells in 100-250 uL FACS buffer of 1.5% paraformaldehyde.
Results Summary:	Clearly and consistently labels CD19+ peripheral blood mononuclear cells.
Additional Notes:	This clone (6D5) works the best of all other BioLegend mouse CD19 clones.

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Publication Status This data has not been published.

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