Product: Purified anti-mouse TNF-α |
Catalog No.: 510802 |
youn Zhai, Research Associate Loyola University Chicago |
Antibody for ELISA to Test Mouse TNF-α | |||
Overall: | Product Quality: | Ease of Use: | |
Anti-mouse TNF-α antibody worked very well for ELISA. |
Experimental Design |
Application: | Test secretion of TNFa in mouse cell line. |
Cells used: | B6 macrophages |
Brief Protocol: | Cells were plated in 24-well plate and treated with LPS overnight. After that, cell culture supernatant was collected and tested for secretion of TNFa by ELISA. |
Results Summary: | LPS induced TNFa secretion in macrophages |
Additional Notes: | The antibody product used in this ELISA is sensitive. |
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Technical Service Notes |
For the x-axis, the concentrations are listed in pg/ml. |
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