Product: Brilliant Violet 510™ anti-human CD14 |
Catalog No.: 301841 |
Katie Nicholas Vowell, Postdoctoral Fellow Vanderbilt University Medical Center |
Brilliant Violet 510™ anti-human CD14 Works Well! | |||
Overall: | Product Quality: | Ease of Use: | |
CD14-BV510 works great to detect monocytes in human PBMC samples. |
Experimental Design |
Application: | Fluorescence cytometry |
Cells used: | Cryopreserved human PBMC |
Brief Protocol: | Cyropreserved human PBMC were thawed, washed twice in PBS, and stained with various volumes of CD14-BV510 in a single color titration. 1 million cells in 200uL PBS were incubated with CD14-BV510 for 15 minutes at room temperature. 1uL CD14-BV510 yielded the highest stain index, however, 0.5uL detected the same frequency of monocytes with only a slightly lower MFI and we have chosen to use that volume going forward to conserve costs since we are only using it in our dump channel to gate out monocytes. |
Results Summary: | 0.5uL CD14-BV510 works great to detect human monocytes. |
Additional Notes: | It is evident in the attached figure that CD14-BV510 clearly marks monocytes, which have higher side scatter properties than other PBMC subsets. |
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