Product: Brilliant Violet 650™ anti-human CD14 |
Catalog No.: 301836 |
Joy Hsu, Specialist UCSF |
Anti-human CD14 Antibody Brilliant Violet 650™™ | |||
Overall: | Product Quality: | Ease of Use: | |
CD14 stain for macrophage is dim but functional. |
Experimental Design |
Application: | flow cytometry |
Cells used: | human tumor infiltrate |
Brief Protocol: | Cell suspension was plated in 96-well plate at 2-4 million cells/ well. Antibody was added in proper flow buffer at 1:100 for 30 minutes in the dark at 4 degrees Celsius. Used in conjunction with anti-human HLA-DR, CD16, CD11c in order to parse out tumor associated macrophages from stimulatory dendritic cells. |
Results Summary: | Not a bright staining but definitely serves the purpose. |
Additional Notes: |
|
|
See all Reviews |
Follow Us