Product: PE Annexin V |
Catalog No.: 640907 |
Jen Barr, Postdoctoral Research Scholar University of Iowa |
Annexin V PE | |||
Overall: | Product Quality: | Ease of Use: | |
Works great for determining apoptotic cells. Would recommend use with 7-AAD. |
Experimental Design |
Application: | Determination of Apoptosis in cultured cells (WT v KO) |
Cells used: | Cultured splenocytes |
Brief Protocol: | Culture splenocytes with anti-CD3 and anti-CD28 stimulation. Harvest cells and perform surface staining for 30 minutes at 4 C in the dark. Wash cells with PBS. Resuspend in Annexin V binding buffer + 5uL Annexin V PE. Mix and incubate 15 minutes in the dark at RT. Add 400ul Annexin V binding buffer and run on flow cytometer. |
Results Summary: | Easily detected Annexin V positive cells. |
Additional Notes: |
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