Application: |
Flow Cytometry |
Cells used: |
Cell suspensions from mouse peripheral blood |
Brief Protocol: |
We isolated cells from peripheral blood of C57BL/6 mice. Red blood cells were lysed and cells were stained using PE anti-mouse CD19 antibody. We stain in a 50ul volume and add PE anti-mouse CD19 diluted at 1/250 final in FACS buffer for 1h at room temperature. |
Results Summary: |
PE anti-mouse CD19 provides a strong and reliable signal that allows staining to study B cells in circulation. |
Additional Notes: |
Figure shows results from cells circulating in the blood from wild type C57BL6 mice gated on CD45+ cells. |
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