Product: APC/Cyanine7 anti-mouse/human CD11b

Catalog No.: 101226

Andreia Lino, PhD Deutsches Rheuma-Forschungszentrum, a Leibniz Institute

CD11b APC/Cyanine7 for Flow Cytometry
Overall:	Product Quality:	Ease of Use:
APC/Cyanine7 anti-mouse/human CD11b is a fairly good antibody.

Experimental Design

Application:	Flow cytometry
Cells used:	Mouse bone marrow
Brief Protocol:	Resuspend in PBS/BSA and count the cells. Load up to 5x106 cells onto a V-Bottom 96-well-plate. Spin down, resuspend in 50uL of PBS/BSA containing αFcγR (2.4G2) 30 µg/ml and incubate 15min at 4°C. Spin down and resuspend in 50 µl of antibody staining solution containing CD11b APC-Cyanine7 (1:200). Incubate 15min at 4°C in the dark. Wash with PBS/BSA, spin down resuspend and transfer to FACS tubes to read. Use PI to exclude the dead cells.
Results Summary:	Macrophages expressed CD11b.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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