Product: APC anti-mouse CD24 |
Catalog No.: 101813 |
Javier Carrero, Assistant Professor Washington University School of Medicine |
Solid CD24 Detection in Lymph Nodes | |||
Overall: | Product Quality: | Ease of Use: | |
Detects both B cells and myeloid cells positive for CD24 in peripheral lymphoid organs. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Lymph nodes |
Brief Protocol: | 1) Isolated cells and made single cell suspension using collagenase/DNAse I. 2) Washed cells with wash buffer (PBS, 1% bovine serum albumin). 3) Blocked Fc receptors with anti-CD16/32 (clone 2.4G2) at 500ng/mL in wash buffer at a final volume of 100 microliters per million cells. 4) Washed cells with wash buffer. 5) Resuspended cells in 100 microliters and added 0.5 microliters of antibody (1:200) per million cells. Mixed well and incubated at 4 degrees centigrade for 30 minutes. 6) Washed cells twice with wash buffer. 7) Added 2% paraformaldehyde in PBS to fix the cells. 8) Washed with wash buffer once. 9) Kept at 4 degrees centigrade until ready to analyzed (up to 24 hours). All steps done in a 96 well V-bottom plate. |
Results Summary: | We detect an average of 4 logs shift in most MHC II+ cells in the lymph nodes. |
Additional Notes: |
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Technical Service Notes |
Enzymatic treatment of samples may affect your epitopes and the antibody's ability to recognize them. |
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