Product: APC/Cyanine7 anti-mouse/human CD44

Catalog No.: 103028

Elodie Mohr, Dr University of Geneva

APC/Cyanine7 anti-CD44 for Flow Cytometry
Overall:	Product Quality:	Ease of Use:
Bright tandem dye.

Experimental Design

Application:	Flow cytometry
Cells used:	Draining lymph nodes from ovalbumin/alum-immunized mouse transferred with ovalbumin-specific CD45.1+ CD4 T cells (OTII)
Brief Protocol:	Three millions cells were prepared in FACS buffer (PBS/Bovine Serum Albumin 1% / 0.1% azide) were plated in round-bottom 96-well plates. Cells were spin down and treated with Fc block (5% of supernatant from a 2.4 G2 hybridoma culture in FACS buffer), for 10 min at 4°C. Cells were spin down and stained in 25 µl of FACS buffer with APC/Cyanine7 anti-CD44 (1:200), BV510™ anti-CD45.1 and BV421™ anti-CD4 during 20 min at 4°C in the dark. Cells were washed twice with 150 µl of FACS buffer PBS/BSA, and resuspended in FACS buffer for analysis by flow cytometry.
Results Summary:	activated OTII cells stain positive (grey histogram) while OTII from naïve mouse are negative (dashed histogram)
Additional Notes:	T cell activation/memory marker for flow cytometry. Easy to use but the compensations with APC needs to be checked.

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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