Product: Brilliant Violet 421™ anti-mouse/human CD11b

Catalog No.: 101236

Andrew Borkowski, Research Associate TSRI

CD11b Stain of Skin Cells
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Epidermal cells were stained for flow cytometric analysis.

Experimental Design

Application:	Flow cytometry
Cells used:	Mouse epidermal cells
Brief Protocol:	Epidermis and dermis were separated using trypsin. Epidermis was then digested with trypsin. Cell suspensions were filtered and incubated in complete RPMI media overnight prior to staining with anti-mouse CD11b-Brilliant Violet 421 the following day for use in a flow cytometry experiment.
Results Summary:	Specific populations of cells in the epidermis stained positive for this antibody.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

Technical Service Notes

Trypsin treatment of samples can affect some epitopes, making it difficult for antibodies to recognize them.

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