Product: Brilliant Violet 421™ anti-mouse/human CD11b

Catalog No.: 101236

Elodie Mohr, Dr University of Geneva

Brilliant Violet 421™ anti-mouse CD11b for Flow Cytometry
Overall:	Product Quality:	Ease of Use:
Neat and easy staining for myeloid cells.

Experimental Design

Application:	Flow cytometry
Cells used:	Splenocytes from naive C57Bl/6 mouse
Brief Protocol:	Three millions splenocytes prepared in FACS buffer (PBS/Bovine Serum Albumin 1% / 0.1% azide) were plated in round-bottom 96-well plates. Cells were spin down and treated with Fc block (5% of supernatant from a 2.4 G2 hybridoma culture in FACS buffer), for 10 min at 4°C. Cells were spin down and stained in 25 µl of FACS buffer with BV421™ anti-CD11b (1:1300) during 20 min at 4°C in the dark. Cells were washed twice with 150 µl of FACS buffer PBS/BSA, and resuspended in FACS buffer for analysis by flow cytometry.
Results Summary:	Identification of MHC class II negative granulocytes and monocyte/macrophages and a subset of MHC Class II positive dendritic cells among TCRbeta negative/IgM negative gate.
Additional Notes:	Identification of myeloid cells and B1 cells by flow cytometry.

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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