Product: Brilliant Violet 605™ anti-human CD45RA |
Catalog No.: 304134 |
Brahma Kumar, Graduate Student Columbia University |
Very Good CD45RA Antibody (Brilliant Violet 605™) | |||
Overall: | Product Quality: | Ease of Use: | |
This anti-human CD45RA gives good separation for easy identification of T cell subsets. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | splenocytes, PBMCs, lung |
Brief Protocol: | 30 min incubation on ice in the dark in 100ul in PBS containing 1% FBS and azide. Antibody used at 1:100. Optimal staining at 1:50 but still good separation at 1:200. |
Results Summary: | Images show T cells isolated from spleen, one image for CD4 one for CD8, shown vs CCR7. |
Additional Notes: |
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