Product: Alexa Fluor® 700 anti-human CD158e1 (KIR3DL1, NKB1)

Catalog No.: 312712

Quirin Hammer, M Sc German Rheumatism Research Center

Staining of CD158e1 (KIR3DL1) on human NK Cells (Alexa Fluor® 700)
Overall:	Product Quality:	Ease of Use:
Clear separation of KIR3DL1+ and KIR3DL1- NK cells.

Experimental Design

Application:	Flow cytometry
Cells used:	Human PBMC
Brief Protocol:	Freshly isolated human PBMC cells were washed with PBS, stained for 15 minutes at room temperature in the dark, washed two times with PBS, fixed in 2% paraformaldehyde solution for 15 minutes at room temperature in the dark, washed two times with PBS/BSA and subsequently analyzed at a BD LSRFortessa flow cytometer. Gated on viable (fixable viability dye-) CD14- CD19- CD3- CD56dim NK cells.
Results Summary:	Clear separation of KIR3DL1+ and KIR3DL1- NK cells.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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