Our multiplex immunoassays are ideal for all your research needs. Our flow cytometry-based multiplex immunoassays are optimized to quantitate up to 14 analytes. Choose from our pre-defined panels designed for your research area or customize our panels for your analytes of interest.


LEGENDplex™ allows researchers users to quantify cytokines on most common flow cytometers. It utilizes beads pre-coated with antibodies for their intended target. With two bead sizes and distinct intrinsic levels of APC fluorescence, bead populations to easily be identified in our complimentary software suite. Once the analyte is bound, a cocktail of biotinylated antibodies is added. This is followed by PE-Streptavidin, which serves as the signal to help quantify cytokine concentrations alongside a standard curve. 

The ability to detect up to 14 analytes with increased sensitivity and dynamic range and as little as 25 µL of sample speeds up discoveries and breakthroughs. Explore our available product listings, software support, and resources below.

Instrument Setup and Data Analysis

To simplify data analysis, we provide the LEGENDplex Data Analysis Software Suite, a free cloud-based program for flow cytometry data file analysis, and helpful information on flow cytometer instrument setup.

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Explore our resources including protocols, blogposts and videos on LEGENDplex. Learn about the technology and how it can drive your discoveries.

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Pre-defined Panels

Find our pre-defined panels for flow cytometry, build custom panels with target analytes, and get your ancillary reagents for your multiplex immunoassays.

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Panel Wizard

If you would prefer to browse our LEGENDplex analytes first, use our Panel Wizard to see if your ideal panel already exists. If it needs to be created, get in touch with our Custom Solutions Team.

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