BioLegend provides a wide selection of antibodies validated and quality-control tested for a variety of applications including Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), or Chromatin IP (ChIP). Targets include a wide selection of cell biology proteins including cell signaling, cell structure, cell development and differentiation, cell proliferation and growth, cell cycle, epigenetic and transcriptional regulators, cell adhesion, and cell death proteins, as well as epitope tags.


Application Protocols


BioLegend’s Commitment to Antibody Quality

 

To ensure the highest quality of products, BioLegend’s cell biology antibodies undergo extensive validation processes, which include:

 

  • Testing on knockout (KO) or knockdown (KD) cell lysates (CRISPR/Cas9 or siRNA), and negative control cell lines to confirm antibody specificity.
  • Testing on multiple species (human, mouse, rat, yeast, bacteria, zebrafish) whenever applicable, to determine antibody cross-reactivity.
  • Side-by-side comparison testing with competitor’s antibody to ensure sensitivity.
  • Side-by-side comparison testing of lots with internal controls for lot-to-lot consistency.
  • Clones for one application (such as WB or ChIP) further validated in multiple applications (such as microscopy, flow cytometry) to provide the user the flexibility of supplementing their data with additional assays specific to their research.
  • Optimization of antibody suggested use by a titration range.

Go-ChIP-Grade™ Protein G Enzymatic Kit

BioLegend offers a variety of products to help perform effective ChIP assays. We offer high-quality Go-ChIP-Grade™ Purified Antibodies for ChIP assays directed toward diverse targets including histone modifications, chromatin-associated proteins, and transcription factors. We also offer a Go-ChIP-Grade™ Protein G Enzymatic Kit for a quicker, easier, and more efficient way to perform ChIP assays.

 


 

Direct-Blot™ HRP Antibodies

When performing western blots, it is common to use a secondary antibody to detect the proteins of interest. To help you perform western blots with ease, BioLegend offers Direct-Blot™ primary antibodies directly conjugated to horseradish peroxidase (HRP).


 

Western-Ready™ ECL Substrate

 

  • ECL substrate with high sensitivity
  • A luminol-based detection system
  • Suitable for CCD imager and film
  • Available in two sizes for 10 or 100 mini-blots
  • Signal duration up to 4 hours

 

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Prime-Step™ Prestained Broad Range Protein Ladder

The Prime-Step™ Prestained Broad Range Protein Ladder is a three-color protein standard with 10 prestained chromophore-conjugated recombinant proteins covering a wide range of molecular weights, from 6.5-270 kDa. It is ideal for monitoring protein separation during SDS-PAGE, confirming WB transfer on membranes, and estimating protein sizes.

data image

 

Cell Biology Antibody Sampler Kits

BioLegend offers Cell Biology Antibody Sampler Kits as a convenient and economical way to test multiple targets. These kits target various protein groups including epitope tags, HDACs, IRFs, and many others…

Current Kits include:

  • E. coli RNA Polymerase Antibody Sampler Kit
  • Epitope Tag Big Motif Antibody Sampler Kit
  • Epitope Tag Big Motif Antibody Sampler Kit
  • HDAC Antibody Sampler Kit
  • IRF Antibody Sampler Kit

 

Below are reviews from customers collected at BioLegend and other external sites for our Cell Biology products. To browse and search all the reviews, visit our Reviews Library.

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BioLegend's Cell Biology products have been extensively published. Browse and search the full Publication Library.

Product Authors Journal Year Vol/Page Pubmed
Purified anti-Acetylated Lysine Lee J, Homma T, Fujii J, et al. Toxicol Lett 2017 10.1016/j.toxlet.2017.06.005 Link
PE anti-AIM2 Svensson A, et al. PLoS One. 2017 10.1371/journal.pone.0183268 Link
Purified anti-AIM2 Su S Cell 2018 175:442 Link
PE anti-mouse Aiolos Lee CG, et al. JCI Insight 2019 4:5 Link
Purified anti-Alix Yang C, et al. PLoS One 2011 6:e22517 Link
Purified anti-Alix Morel L, et al. J Biol Chem 2013 288:7105 Link
Purified anti-Alix Ertunc M, et al. J Lipid Res 2015 56:423 Link
Purified anti-Alix Bæk R, et al. J Immunol Methods 2016 438:11-20 Link
Purified anti-Alix Gärtner K, et al. Cancer Med 2018 11:39 Link
Purified anti-Alix Schiller LT, et al. Mol Ther Methods Clin Dev 2018 15:1630 Link
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