Fluorophore Ex (nm) Max Em (nm) Max Filter Used Brightness
PE 565 575 585/20 5
PE/Cyanine5 565 670 660/20 5
PE/Dazzle™ 594 565 610 610/20 5
PE/Fire™ 700* 565 695 - 5
Alexa Fluor® 647 650 668 660/20 4
APC 650 660 660/20 4
Brilliant Violet 421™ 405 421 450/50 4
Brilliant Violet 711™ 405 711 710/50 4
PE/Cyanine7 565 774 780/60 4
PE/Fire™ 640* 565 639 - 4
PE/Fire™ 810* 565 806 - 4
APC/Fire™ 810* 650 807 - 3
Brilliant Violet 605™ 405 603 610/20 3
Brilliant Violet 650™ 405 645 660/20 3
Brilliant Violet 750™ 405 750 780/60 3
Brilliant Violet 785™ 405 785 780/60 3
Spark YG™ 593* 574 593 - 3
KIRAVIA Blue 520™ 488 520 530/30 3
Alexa Fluor® 700 696 719 720/45 2
APC/Cyanine7 650 774 780/60 2
APC/Fire™ 750 650 774 780/60 2
PerCP/Cyanine5.5 482 690 695/40 2
Spark NIR™ 685* 660 685 - 2
Spark YG™ 581* 565 581 - 2
Alexa Fluor® 488 495 519 530/30 1
Brilliant Violet 510™ 405 510 510/50 1
Brilliant Violet 570™ 405 570 585/42 1
FITC 493 525 530/30 1
Pacific Blue™ 410 455 450/50 1
PerCP 482 675 695/40 1
Spark Blue™ 550* 516 540 - 1
Spark Violet™ 538 399 538 - 1

*Dyes denoted by an asterisk are recommended to be used on spectral unmixing flow cytometers. They may be compatible with conventional flow cytometers, but end users may need to optimize filter sets.

The Fluorophore Brightness Index Score is a relative indication of fluorescence intensity above the background for each fluorophore antibody conjugation (1=dim, 5=brightest). These values can differ depending upon the flow cytometer, the instrument filters and settings, laser power, the antibody clone and the antigen target, f/p ratios, the buffer conditions, etc.

 

 

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