About the webinar

This joint webinar was co-hosted by 10x Genomics, Illumina, and BioLegend, and features Dr. Andrew Martins, Assistant Professor, Immunobiology, Yale School of Medicine.

Human immune responses are often monitored via phenotyping peripheral blood mononuclear cells (PBMC), which is a heterogenous mixture of immune cell types. Traditional approaches to assess immune states in PBMC include cell subset quantification by flow cytometry and expression profiling with bulk RNA microarray or RNA-Seq. With the advent of multimodal single-cell RNA-Seq approaches, such as those that combine the detection of antibodies directed against surface antigens with the capture of intracellular mRNA, it has become possible to combine these approaches and allow cell surface marker-resolved subset phenotyping with unbiased cell state assessment by RNA-Seq. This presentation will cover the experimental strategy utilized to study circulating immune cell states in relation to vaccine responsiveness and the disease course of severe COVID-19, the advantages and pitfalls of the approach, and a look toward the future of human immune state profiling.