Recombinant Human CXCL16 (carrier-free)

Pricing & Availability
Other Names
Small-inducible cytokine B16 (SCYB16), Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein (SRPSOX)
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CXCL16_Human_RECOM_BA_052716
Baf3-hCXCR6 transfectants attracted by human CXCL16
  • CXCL16_Human_RECOM_BA_052716
    Baf3-hCXCR6 transfectants attracted by human CXCL16
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Cat # Size Price Quantity Avail. Save
591002 10 µg 88€
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591004 25 µg 156€
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591006 100 µg 468€
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591008 500 µg 1 220€
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Description

CXCL16 is a transmembrane chemokine and ligand for CXCR6 that was initially identified by bioinformatics. CXCL16 is a non-ELR CXC chemokine, and it contains a 110 amino acid spacer region, a transmembrane domain, and a cytoplasmic tail. The spacer domain is similar to a region present in CX3CL1. This region is rich in serine, threonine, proline, and highly glycosylated: a characteristic distinctive of mucin structures. Soluble CXCL16 is present in supernatants of macrophages and is upregulated by LPS and TNFα treatment. ADAM10 participates in the constitutive shedding of transmembrane chemokines (CXCL16 and CX3CL1). Treatment of cells expressing CXCL16 or CX3CL1 with PMA and ionomycin increases the shedding of these chemokines. ADAM17 seems to participate in the PMA-induced shedding of CXCL16 in glial cells; in keratinocytes, ADAM10, but not ADAM17, participates in the shedding. CXCL16 and CXCR6 play key roles in tumor progression and bone metastasis in prostate cancer. In this system, CXCL16 recruits MSC mesenchymal stem cells (MSC) (under activation) are differentiated to cancer associated fibroblasts (CAFs) which secrete CXCL12, which subsequently promotes metastasis. CXCL16 has been detected in clinical samples of lymphoma patients. Also, CXCL16 participates in bone homeostasis; TGF-β1 induces CXCL16 and LIF production in osteoclasts, which regulate recruitment of osteoblasts to re-establish the bone lost during the resorptive phase of bone turnover.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human CXCL16, amino acids (Asn49-Pro137) (Accession# M_001100812.1) was expressed in E. coli.
Molecular Mass
The 89 amino acid recombinant protein has a predicted molecular mass of approximately 10 kD. The DTT-reduced and non-reduced protein migrate at approximately 13 and 14 kD by SDS-PAGE respectively. The N-terminal amino acid is Asn.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1% BSA or HSA. After dilution, the cytokine can be stored between 2°C and 8°C for one month or from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Bioactivity was measured by its property to chemoattract Baf3-hCXCR6 transfectants in a dose dependent manner. The ED50 = 0.5 — 3 ng/ml.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution
B cells, monocytes, macrophages, splenic and lymph node dendritic cells, bronchial epithelial cells, smooth muscle cells, keratinocytes, glioma cells, astrocytes.
Function
CXCL16 plays a role in T cell-DC interaction and movement of activated T cells in the splenic red pulp region. CXCL16 also functions as a scavenger receptor for oxidized low density lipoproteins. CXCL16 expression is induced by inflammatory mediators (IL-1β, TNFα, IFNγ).
Interaction
Intraepithelial lymphocytes (IELs), natural killer T (NKT) cells, activated CD8 and CD4 T cells, human gingival fibroblasts, and mesenchymal stem cells.
Ligand/Receptor
CXCR6 (CD186).
Cell Type
Hematopoietic stem and progenitors
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References

1. Matloubian M, et al. 2000. Nat. Immunol. 1:298.
2. Wilbanks A, et al. 2001. J. Immunol. 166:5145.
3. Able S, et al. 2004. J. Immunol. 172:6362.
4. Ludwig A, et al. 2005. J. Neurochem. 93:1293.
5. Hundhausen C, et al. 2007. J. Immunol. 178:8064.
6. Scholz F, et al. 2007. J. Invest. Dermatol. 127:1444.
7. Hosokawa Y, et al. 2009. Clin. Exp. Immunol. 156:413.
8. Jung Y, et al. 2013. Nat. Commun. 4:1795.
9. Ota K, et al. 2013. Bone 57:68.

Gene ID
58191 View all products for this Gene ID
UniProt
View information about CXCL16 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 4    Revision Date: 09.19.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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