ELISA MAX™ Standard Set Mouse IL-1β

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Regulatory Status
Other Names
IL-1b ELISA kit
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Product Citations
mouse il-1b_122109
  • mouse il-1b_122109
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432601 5 plates 224,00€
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The 10- and 20-plate sizes of this assay have been discontinued. The assay will remain available for purchase under the 5-plate catalog number. Please contact your BioLegend sales representative for bulk pricing quotations.

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse IL-1β ELISA MAX™ Capture Antibody (200X)
  • Mouse IL-1β ELISA MAX™ Detection Antibody (200X)
  • Mouse IL-1β Standard
  • Avidin-HRP (1000X)

Product Details



As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application Notes

BioLegend's mouse and human IL-1ß ELISA kits use antibodies raised against the mature form of IL-1ß. However, because pro-IL-1ß contains the mature form, these antibodies likely recognize both the pro- and the mature forms of the protein. The human IL-1ß ELISA kits have been shown to equally recognize both pro- and mature forms of IL-1ß.

Application References

(PubMed link indicates BioLegend citation)
  1. O'Sullivan BJ, et al. 2006. J. Immunol. 176:7278.
  2. Murayama MA, et al. 2014. Biochem Biophys Res Commun. 443:42. PubMed
  3. Rapsinski GJ, et al. 2015. Infect Immun. 83:693. PubMed
Product Citations
  1. Kolar SL, et al. 2019. JCI Insight. 4:5. PubMed
  2. Li X, et al. 2019. Immunity. 50:591. PubMed
  3. Segovia M, et al. 2019. Cancer Cell. 35:767. PubMed
  4. Chen C, et al. 2017. Mol Med Rep. 15:2289. PubMed
  5. Batista SJ, et al. 2020. Nat Commun. 3.01875. PubMed
  6. Wong MY, et al. 2020. J Neuroinflammation. 0.841666667. PubMed
  7. Murayama M, et al. 2014. Biochem Biophys Res Commun. 443:42. PubMed
  8. Rapsinski G, et al. 2015. Infect Immun . 83:693. PubMed
  9. Brown P, et al. 2015. PLoS One. 10:125126. PubMed
  10. Andrade-Sousa A, et al. 2016. PLoS One. 11: 0163420. PubMed
  11. Th?nert R, et al. 2017. Nat Commun. 8:14268. PubMed
  12. Guo Y, et al. 2020. J Clin Invest. 130:6301. PubMed
  13. Tan EE, et al. 2020. J Clin Invest. 130:5817. PubMed
  14. Santos I, et al. 2020. Proc Natl Acad Sci U S A. 117:12281. PubMed
  15. Theivanthiran B, et al. 2020. J Clin Invest. 130:2570. PubMed
  16. Xu J, et al. 2020. J Am Soc Nephrol. 31:54:00. PubMed
  17. Jung S, et al. 2021. Endocrinology. 162(3):. PubMed
  18. Li Y, et al. 2021. Cardiovasc Res. 890:117. PubMed
  19. Li H, et al. 2021. Mol Med Rep. 1:23. PubMed
AB_2883996 (BioLegend Cat. No. 432601)
Standard Range
31.3-2,000 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Coating Buffer: 8.4 g NaHCO3, 3.56 g Na2CO3, add DI H2O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
  • Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
  • PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
  • Wash bottle or automated microplate washer
  • TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
  • Stop Solution (2 N H2SO4)
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

Antigen Details

Cell Sources
Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Gene ID
16176 View all products for this Gene ID
View information about IL-1beta on UniProt.org

Related FAQs

Do your IL-1β ELISA kits detect pro-IL-1β or the mature form?

The antibodies in our IL-1β kits were generated against the mature form of the protein. However, because the pro-form of the protein contains the mature form, the antibodies should detect both forms.

Go To Top Version: 3    Revision Date: 09.11.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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