- SMI 22 (See other available formats)
- Regulatory Status
- Other Names
- Glial fibrillary acidic protein
Covance Catalog# SMI-22R
- Mouse IgG2b
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- Product Citations
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Glial fibrillary acidic protein is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes and ependymal cells. GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts, Leydig cells of the testis, keratinocytes, osteocytes and chondrocytes and stellate cells of the pancreas and liver. GFAP is a type III IF protein that is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells.
Type III intermediate filaments are highly conserved and contain three domains, named the head, rod and tail domains. This rod domain coils around that of another filament to form a dimer, with the N-terminal and C-terminal of each filament aligned. Type III filaments such as GFAP are capable of forming both homodimers and heterodimers; GFAP can polymerize with other type III proteins or with neurofilament protein (NF-L). Interestingly, GFAP and other type III IF proteins cannot assemble with keratins, the type I and II intermediate filaments: in cells that express both proteins, two separate intermediate filament networks form.
To form networks, the initial GFAP dimers combine to make staggered tetramers, which are the basic subunits of an intermediate filament. The non-helical head and tail domains are necessary for filament formation. The head and tail regions have greater variability of sequence and structure. In spite of this increased variability, the head of GFAP contains two conserved arginines and an aromatic residue that are required for proper assembly.
- Human, Mouse, Rat
- Antibody Type
- Host Species
- Ascites Fluid (contains 0.01M sodium azide).
- The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
- Storage & Handling
- Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
IHC-P - Quality tested
ICC, WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:1000 – 1:2000 is suggested. For western blotting, a dilution range of 1:1000 – 1:2000 is suggested. For immunocytochemistry, a dilution range of 1:1000 – 1:2000 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Multiple protein fragments ranging from 38 to 48 kD have been reported in human CNS lysates resulting from caspase- and calpain-mediated cleavage of GFAP.
(PubMed link indicates BioLegend citation)
- Product Citations
AB_2565344 (BioLegend Cat. No. 835301)
- GFAP is a 432 amino acid protein with a molecular mass of ~50 kD.
Tissue distribution: GFAP is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, ependymal cells, and Bergmann glia cells (protoplasmic astrocyte). GFAP is expressed in cells lacking fibronectin.
Cellular distribution: Cytoskeleton and cytosol
- GFAP is a class-III intermediate filament and a structural constituent of the cytoskeleton. It is a cell-specific marker that is used to distinguish astrocytes from other glial cells during the development of the CNS.
- Cell Type
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Antigen References
- van Bodegraven EJ, et al. 2019. Glia. 10.1002/glia.23594.
- Pekny M, et al. 2019. Neurosci Lett. 689:45.
- Hol EM, et al. 2017. Cold Spring Harb Perspect Biol. 9(12)
- Gene ID
- 2670 View all products for this Gene ID
- View information about GFAP on UniProt.org
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