Veri-Cells™ CD4-Low PBMC

Kit Contents

Kit Contents

Cat. No. 425601

• 1 vial of lyophilized Veri-Cells™ CD4-Low PBMC (≥ 5 x 10⁶ cells)
• 1 vial containing 1.5 ml of Veri-Cells™ Reconstitution Buffer A Plus

Cat. No. 425602

• 4 vials of lyophilized Veri-Cells™ CD4-Low PBMC (≥ 5 x 10⁶ cells per vial)
• 4 vials containing 1.5 ml of Veri-Cells™ Buffer A Plus each
   

Veri-Cells™ Buffer A Plus is an improved version of the original Veri-Cells™ Buffer A to improve staining performance with tandem dyes.

Product Details

Formulation

Each vial of Veri-Cells™ CD4-Low PBMC contains ≥ 5 x 10⁶ lyophilized human peripheral blood mononuclear cells (PBMC).

Preparation

The Veri-Cells™ CD4-Low PBMC Kit is prepared from lyophilized human peripheral blood mononuclear cells and a vial of reconstitution buffer.

Storage & Handling

Store the kit at 2°C - 8°C upon receipt. Do not open sealed pouch until ready to use. Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided. Contact BioLegend Technical Support for information on the current lots.

Application

FC - Quality tested
ICFC - Verified

Recommended Usage

50 µL per test (after reconstitution with 1.3 ml)

Once reconstituted, let the cells sit for 15 minutes at room temperature prior to staining.

Veri-Cells™ Reconstitution Buffer A Plus may show precipitation over time, however this is normal and does not affect performance of the buffer.

Application Notes

The Veri-Cells™ CD4-Low PBMC Kit can be used as positive controls for surface and intracellular immunofluorescent staining and multi-color flow cytometry assays. Assay values for CD3⁺CD4⁺ cells will be provided for each lot produced. It can also be used for the detection of intracellular molecules such as Granzyme B, Perforin, Foxp3 and Helios using BioLegend’s Fixation Buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (10X) (Cat. No. 421002) or the True Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). For best results, Cell Staining Buffer (Cat. No. 420201) is recommended for any washing steps. Please note, any claims relative to product performance are based on testing with BioLegend reagents and protocols. Deviations from the use of these reagents and procedures have not been assessed and may affect performance.
 

Cell subset	Acceptance Criteria, % of lymphocyte gate
CD4+CD3+	8-18

Note on indicated ranges: Percent positive values were determined by gates set on unstained cells. The antibodies used for testing were the following: CD3 FITC (clone UCHT1) and CD4 PE/Cy7 (clone RPA-T4). Variability in antibodies used, instruments, staining conditions and other factors may influence end-user values. The Certificate of Analysis specific values are provided as a reference. It is recommended that individual laboratories establish its own control values and ranges for each parameter based on laboratory specific conditions and protocols.

Please use our CoA Look-Up Tool to find the expected lot-specific frequencies of certain cell populations included within each lot of Veri-Cells™ CD4-Low PBMC. The antibodies used for testing were the following: CD3 FITC (clone UCHT1) and CD4 PE/Cy7 (clone RPA-T4).  Percent positive is based on gates set on unstained cells. The Certificate of Analysis specific values are provided only as a reference. Variability in antibodies used, instruments, staining conditions and other factors may influence end-user values. It is recommended that individual laboratories establish its own control values and ranges for each parameter based on laboratory specific conditions and protocols.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Veri-Cells™ Buffer A Plus is an improved version of the original Veri-Cells™ Buffer A to improve staining performance with tandem dyes.

Antigen Details

Biology Area

Immunology

Molecular Family

CD Molecules

Gene ID

NA

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Protocol for using Veri-Cells™

Related Pages & Pathways

Related FAQs

Is washing required after staining?

Yes, it is recommended to wash after staining. We recommend using BioLegend's Cell Staining buffer (Cat. No. 420201) for the wash step.

Can the cells be activated after rehydration?

No, they cannot be activated.

Are these cells virus/pathogen free?

These are tested to be free of HIV, HBV, syphilis, and HCV.

Are there any RBCs in Veri-cells™?

There may be a few, but the majority of RBCs are not present in Veri-Cells™ preparations.

Are these cells viable?

No they are not.

Can a constant percentage of each population be expected in each lot?

Yes, within each lot, cell populations remain constant.

Are the cells fixed?

They are treated with our proprietary mixture prior to lyophilization.

Which markers can be stained?

160+ known markers have been verified - please view markers verified by LEGENDScreen™ or contact tech@biolegend.com for a complete list.

Can they be used to detect intracellular cytokines such as TNF-α or IFN-γ?

For this, use Veri-Cells™ Activated (Cytokine) PBMC which have been activated with Cell Activation Cocktail (with Brefeldin A) containing PMA and Ionomycin. These have been validated to stain positive for activation-induced cytokines including TNF-α and IFN-γ. Veri-Cells™ PBMC and Veri-Cells™ CD4-Low PBMC will not have detectable TNF-α or IFN-γ.

How do we QC these cells?

Each Veri-Cells™ product is tested with a panel of antibodies to ensure expression of these markers falls within the expected range.  For more information regarding the exact markers used for each product, please contact technical service.

Certificate of Analysis