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ELISA MAX™ Standard Set Human TNF-α

Pricing & Availability
Regulatory Status
RUO
Other Names
TNF-a ELISA Kit
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Product Citations
publications
human tnf-a_121609
  • human tnf-a_121609
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430201 5 plates 212€
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Description

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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human TNF-α ELISA MAX™ Capture Antibody (200X)
  • Human TNF-α ELISA MAX™ Detection Antibody (200X)
  • Human TNF-α Standard
  • Avidin-HRP (1000X)

Product Details

Verified Reactivity
Human
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Davis AM, et al. 2008. J. Immunol. 180:6923. PubMed
  2. Kulkarni O, et al. 2009. J. Pharmacol Exp Ther. 328:371. PubMed
  3. Wang X, et al. 2014. Int J Cardiol. 174:688. PubMed
  4. Sautto GA, et al. 2015. Gut. PubMed
Product Citations
  1. Wang X, et al. 2014. Int J Cardiol. 174:6888. PubMed
  2. Sautto G, et al. 2015. Gut. . PubMed
  3. Bibby J, et al. 2020. Nat Commun. 2.827777778. PubMed
  4. Zeng W, et al. 2021. Exp Ther Med. 22:1186. PubMed
  5. Zhou J, et al. 2021. Front Immunol. 12:656242. PubMed
  6. Chen S, et al. 2021. Exp Ther Med. 22:1049. PubMed
  7. Kong B, et al. 2017. J Neuroinflammation. 10.1186/s12974-017-0961-z. PubMed
  8. Davis A, et al. 2008. J Immunol. 180:6923. PubMed
  9. Bikker R, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.06.009. PubMed
  10. Palacios Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  11. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  12. Veerasubramanian PK, et al. 2021. Bioengineering (Basel). 9:. PubMed
  13. Nakatomi K, et al. 2021. Biomed Rep. 14:21. PubMed
  14. Ban T, et al. 2021. Nat Commun. 12:4379. PubMed
  15. Qiu J, et al. 2010. Appl Envir Microbiol. 76:5846. PubMed
  16. Hoang QTM, et al. 2021. Front Med (Lausanne). 8:676058. PubMed
  17. Mamriev D, et al. 2020. Cell Death Dis. 0.79375. PubMed
  18. Yu Y, et al. 2021. Cell Rep. 37:110126. PubMed
  19. Sohail A, et al. 2022. PLoS Pathog. 18:e1010219. PubMed
  20. Grech D, et al. 2016. J Acupunct Meridian Stud. 9:234-241. PubMed
  21. Khongkhunthian S, et al. 2018. Drug Discov Ther. 11:323. PubMed
  22. Rana T, et al. 2021. PLoS One. 16:e0246393. PubMed
  23. Jø nsson K, et al. 2017. Nat Commun. 8:14391. PubMed
  24. Kulkarni O, et al. 2009. J Pharmacol Exp Ther. 328:371. PubMed
  25. Sadewasser A, et al. 2019. Mol Ther Nucleic Acids. 16:686. PubMed
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Coating Buffer: 8.4 g NaHCO3, 3.56 g Na2CO3, add DI H2O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
  • Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
  • PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
  • Wash bottle or automated microplate washer
  • TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
  • Stop Solution (2 N H2SO4)
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
7124 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 05.09.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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