- QA16A15 (See other available formats)
- Other Names
- IGHG4, immunoglobulin heavy chain constant region gamma 4
- Human IgG4, κ
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- Product Citations
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mmunoglobulin G4, or IgG4, is a type of antibody. Humans express four types of IgG (IgG1, IgG2, IgG3, and IgG4). Antibodies in mammals are complex proteins, composed of four independent amino acid chains. The molecule is formed by two identical heavy chains and two identical light chains, and produced by B cells. The FcγR molecules (CD16, CD32, and CD64) on the surface of various immune cells can bind IgG1, IgG2, and IgG4.Product Details
- Antibody Type
- Host Species
- 0.2 µm filtered in phosphate-buffered solution, pH 7.8, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Quality tested
Elisa Control - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.
- Application Notes
QA16A15 is a recombinant human IgG4. It can be used as an isotype control when evaluating fully human, humanized, or chimeric antibodies. This product is suitable as an isotype-matched control for ELISA, immunoblotting, FACS, IHC, and functional studies.
- Immunoglobulin, 150 KDa
- Cell Type
- B cells
- Biology Area
- Gene ID
- View information about IgG4 on UniProt.org
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
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