Ultra-LEAF™ Purified anti-mouse / rat IL-1β Antibody

Product Details

Verified Reactivity

Mouse, Rat

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

E. coli -expressed, recombinant mouse IL-1β

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture - Quality tested
ELISPOT Capture, Depletion, IHC-F, IP, Neut, WB - Reported in the literature, not verified in house

 

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2.0 - 6.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-1β recombinant protein ranging from 2000 to 30 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture³: The purified B122 antibody is useful as the capture antibody in a mouse IL-1ß sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5158 antibody (Cat. No. 515801) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.

Certain formats of clone B122 have been reported for in vitro neutralization of IL-1ß bioactivity¹, in vivo cytokine depletion⁵, immunohistochemical staining of frozen sections⁶, Western blotting⁷, and the study of endotoxin shock⁸.

Application References

(PubMed link indicates BioLegend citation)

1. Hogquist K, et al. 1991. J. Immunol. 146:1534.
2. Williams RO, et al. 2000. J. Immunol. 165:7240. (Neut)
3. Brewer J, et al. 1998. J. Immunol. 161:4000. (ELISA and ELISPOT Capture)
4. Rogers HW, et al. 1992. Proc. Natl. Acad.Sci. USA 89:1011.
5. Cantwell AM, et al. 2010. BMC Immunol. 11:29. (Depletion)
6. Dube PH, et al. 2001. Proc. Natl. Acad. Sci. USA 98:10880. (IHC)
7. Babelova A, et al. 2009. J. Biol. Chem. 284:24035. (WB)
8. Lu JY, et al. 2006. Genes Dev. 20:3174. (Neut) PubMed

Product Citations

1. Burke TP, et al. 2020. Nat Microbiol. 0.686111111. PubMed
2. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
3. Viladomiu M, et al. 2021. Cell Host Microbe. 29(4):607-619.e8. PubMed
4. Dani N, et al. 2021. Cell. 184(11):3056-3074.e21. PubMed

RRID

AB_2814403 (BioLegend Cat. No. 503517)
AB_2814404 (BioLegend Cat. No. 503518)
AB_2814402 (BioLegend Cat. No. 503516)
AB_2814399 (BioLegend Cat. No. 503513)
AB_2814400 (BioLegend Cat. No. 503514)
AB_2814401 (BioLegend Cat. No. 503515)

Antigen Details

Structure

Cytokine; 17.5 kD (Mammalian)

Bioactivity

Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages

Cell Sources

Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma,

Cell Targets

B cells, T cells, monocytes

Receptors

Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)

Biology Area

Immunology, Innate Immunity

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.

Regulation

Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α₂-macroglobulin

Gene ID

16176 View all products for this Gene ID

UniProt

View information about IL-1beta on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Active Protocols: Sandwich ELISA - Video
• Sandwich ELISA Protocol

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis