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Ultra-LEAF™ Purified anti-human BMP-10 Antibody

Pricing & Availability
Clone
A17182F (See other available formats)
Regulatory Status
RUO
Other Names
BMP10, Bone morphogenetic protein 10
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17182F_ULEAF_BMP-10_Antibody_1_082319
Recombinant human BMP-10 (Black circles, Cat. No. 751606) induces the proliferation of ATDC5 mouse chondrogenic cells. Anti-human BMP-10 antibody (clone A17182F, purple squares) inhibits the effect of recombinant human BMP-10 at 0.1 µg/mL in ATDC5 cells in a dose dependent manner. The ND50 = 4 - 20 µg/mL.
  • A17182F_ULEAF_BMP-10_Antibody_1_082319
    Recombinant human BMP-10 (Black circles, Cat. No. 751606) induces the proliferation of ATDC5 mouse chondrogenic cells. Anti-human BMP-10 antibody (clone A17182F, purple squares) inhibits the effect of recombinant human BMP-10 at 0.1 µg/mL in ATDC5 cells in a dose dependent manner. The ND50 = 4 - 20 µg/mL.
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618103 100 µg 216€
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618104 1 mg 572€
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Description

Bone morphogenetic protein 10 (BMP-10) was initially cloned from embryonic heart, and expression data suggests that it plays a key role in the trabeculation of the embryonic heart. BMP-10 shares >98% amino acid sequence identity among human, mouse, and rat orthologs. BMPs are members of the transforming growth factor β (TGF-β) superfamily. BMP-10 is synthesized as an inactive precursor pro-BMP10 (∼60 kD) and is processed into active BMP-10 (∼14 kD) by proprotein convertase(s). Furin is the main convertase responsible for the generation of BMP-10. BMPs signal through heterotetrameric complex receptors, which include four type I receptors, ALK1, ALK2, ALK3 and ALK6, and three type II receptors, BMPRII, ActRIIA, and ActRIIB. The ligands bind to the heterotetrameric complex composed of two type II receptors and two type I receptors. Under ligand binding, the type II receptors phosphorylate and activate the type I that stimulate the receptor regulated-Smads. Additionally, a co-receptor (endoglin) binds to ALK1 increasing the signal. BMP-9 and -10 are functionally equivalent ligands of ALK1 in vascular development. Nevertheless, BMP-10 has a unique function in cardiac development and cannot be replaced by BMP-9. However, both ligands have a redundant role in postnatal vascular development. ALK1 and endoglin are specifically expressed in endothelial cells and mutations in these receptors are associated with vascular defects.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human BMP-10
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by the inhibition of proliferation induced by human recombinant BMP-10 (0.1 µg/mL) on ATDC5 mouse chondrogenic cells. ND50 range: 4.0 - 20 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814464 (BioLegend Cat. No. 618103)
AB_2814465 (BioLegend Cat. No. 618104)

Antigen Details

Structure
Homodimer.
Distribution

BMP-10 is abundant in developing and postnatal heart.

Function
BMP-10 has a critical role in heart development, modulates cardiomyocyte proliferation and maturation during cardiac ventricular development, and retinal postnatal vascularization. Myocardin, a transcriptional coactivator, regulates BMP-10 expression
Interaction
Endothelial cells.
Ligand/Receptor
Heterotretrameric receptor formed by two type I receptors (ALK1) and two type II receptors (BMPRII, ActRIIA, and ActRIIB). Endoglin is a correceptor for ALK1.
Cell Type
Endothelial cells
Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Neuhaus H, et al. 1999. Mech. Dev. 80:181.
  2. David L, et al. 2007. Blood. 109:1953.
  3. Llorca O, et al. 2007. J. Mol. Biol. 365:694.
  4. Susan-Resiga D, et al. 2011. J. Biol. Chem. 286:22785.
  5. Ricard N, et al. 2012. Blood. 119:6162.
  6. Huang J, et al. 2012. J. Clin. Invest. 122:3678.
  7. Chen H, et al. 2013. Proc. Natl. Acad. Sci. USA. 29:11887.
  8. Tillet E and Bailly S. 2014. Front Genet. 5:456.
Gene ID
27302 View all products for this Gene ID
UniProt
View information about BMP-10 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 08.23.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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