Ultra-LEAF™ Purified anti-human BDNF Recombinant Antibody

Pricing & Availability
Clone
QA19A71 (See other available formats)
Regulatory Status
RUO
Other Names
Brain-derived neurotrophic factor, ANON2, BLUN2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA19A71_ULEAF_BDNF_Recombinant_Antibody_040921.png
Recombinant human BDNF (black circles) (Cat. No. 788904) induces the proliferation of C6 glioma cells. Ultra-LEAF™ purified anti-human BDNF recombinant antibody (purple squares) (clone QA19A71) inhibits the effect of recombinant human BDNF (at 4 µg/mL) in C6 cells in a dose - dependent manner whereas the isotype control (gray triangles) (Cat. No. 400193) does not have the effect. The ND50 = 1 - 5 µg/mL.
  • QA19A71_ULEAF_BDNF_Recombinant_Antibody_040921.png
    Recombinant human BDNF (black circles) (Cat. No. 788904) induces the proliferation of C6 glioma cells. Ultra-LEAF™ purified anti-human BDNF recombinant antibody (purple squares) (clone QA19A71) inhibits the effect of recombinant human BDNF (at 4 µg/mL) in C6 cells in a dose - dependent manner whereas the isotype control (gray triangles) (Cat. No. 400193) does not have the effect. The ND50 = 1 - 5 µg/mL.
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936803 100 µg 196,00€
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936804 1 mg 520,00€
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Description

Brain-derived neurotrophic factor (BDNF) is the second member of the NGF family of neurotrophic factors (neurotrophins) that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5). BDNF shares approximately 55% amino acid identity with other family members. The amino acid sequence of mature BDNF is identical in all mammals examined. All neurotrophins are non-covalently linked homodimers and initially produced as proneurotrophins. These proneurotrophins have distinct biological activity from their mature counterparts. Mature BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems during development. BDNF participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. BDNF is also a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye and placenta. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Recombinant human BDNF
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by the inhibition of proliferation induced by human BDNF (4 µg/mL) on C6 glioma cells. ND50 range: 1 - 5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2892499 (BioLegend Cat. No. 936803)
AB_2892499 (BioLegend Cat. No. 936804)

Antigen Details

Structure
Non-covalently linked homodimer
Distribution

Ubiquitous with high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis.

Function
Supports the survival of sensory and sympathetic neurons, and also dopaminergic midbrain neurons.
Interaction
Sensory neurons, sympathetic neurons, midbrain dopaminergic neurons.
Ligand/Receptor
TrkB
Cell Type
Neural Stem Cells, Neurons
Biology Area
Neurodegeneration, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Growth Factors
Antigen References
  1. Baloh RH, et al. 1998. Neuron. 21:1291-302.
  2. Masure S, et al. 1999. Eur J Biochem. 266:892-902.
  3. Gardell LR, et al. 2003. Nature Med. 9:1383-9.
  4. Honma Y, et al. 2002. Neuron. 35:267-82.
  5. Banerjee A, et al. 2012. PLoS One. 7:e50098.
  6. Wong LE, et al. 2015. Proc Natl Acad Sci USA. 112:6170-5.
  7. Zhang M, et al. 2016. Oncotarget. 7:3267-82.
Gene ID
627 View all products for this Gene ID
UniProt
View information about BDNF on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 04.09.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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