Recombinant Mouse CSF-1R (CD115)-Fc Chimera (carrier-free)

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Other Names
Macrophage colony-stimulating factor 1 receptor, M-CSFR, M-CSF-R, CSF-1 receptor, CSF-1R, CSF-1-R, CSF1R, Proto-oncogene c-Fms, FMS, CD115
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Mouse_CSF-1R_CD115_CF_RECOM_1_092320.png
Recombinant mouse CSF-1R-Fc chimera inihibits the proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells induced by recombinant mouse M-CSF (100 ng/mL) in a dose-dependent manner. The ED50 range for this effect is 0.05 - 0.2 µg/mL.
  • Mouse_CSF-1R_CD115_CF_RECOM_1_092320.png
    Recombinant mouse CSF-1R-Fc chimera inihibits the proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells induced by recombinant mouse M-CSF (100 ng/mL) in a dose-dependent manner. The ED50 range for this effect is 0.05 - 0.2 µg/mL.
  • Mouse_CSF-1R_CD115_CF_RECOM_2_092320.png
    Stability Testing for Recombinant Mouse CSF-1R-Fc Chimera. Recombinant mouse CSF-1R-Fc chimera was aliquoted in PBS at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in a proliferation assay with M-NFS-60 mouse myelogenous leukemia lymphoblast cells.
Cat # Size Price Quantity Avail. Save
792104 25 µg 140€
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792106 100 µg 476€
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Technical Data Sheet (pdf)

Product Details

Source
Mouse CD115, amino acid Ala20-Ser511 (Accession # P09581.3), with a linker (GS) and a C-terminal mouse IgG2a (Glu98-Lys330), was expressed in 293E cells.
Molecular Mass
The 727 amino acid recombinant protein has a predicted molecular mass of approximately 81.72 kD. The DTT-reduced and non-reduced protein migrates at approximately 115 kD and 240 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Ala.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.05 - 0.2 μg /mL as measured by its ability to inhibit the proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells induced by recombinant mouse M-CSF (100 ng/mL).
Application

Bioassay

Antigen Details

Structure
Disulfide linked homodimer
Distribution

Primitive multipotent hematopoietic cells, monocytes/macrophages, plasmacytoid, conventional dendritic cells, B cells, smooth muscle cells, neuron, osteoclasts and some cancer cells; plasma membrane

Function
CSF-1R is involved in regulation of survival, proliferation and differentiation of hematopoietic progenitor cells. It is also involved in innate immunity and inflammation.
Ligand/Receptor
Macrophage Colony Stimulating Factor (M-CSF) and IL-34
Bioactivity
Measured by its ability to inhibit the proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells
Antigen References

1. Sapi E, et al. 1995. Oncogene. 10: 529.
2. Dai XM, et al. 2002. Blood. 99: 111.
3. Lin EY, et al. 2001. J Exp Med. 193: 727.
4. Gill K, et al. 2001. Fertil Steril. 76: 1005.
5. Flick MB, et al. 2002. J Cell Biochem. 85: 10.
6. Chihara T, et al. 2010. Cell Death Differ. 17: 1917.
7. Chitu V, et al. 2006. Curr Opin Immunol. 18: 39.
8. Wei S, et al. 2010. J Leukoc Biol. 88: 495.
9. Yeung YG, et al. 1998. J Biol Chem. 273: 17128.
10. Chambers SK, et al. 1994. Steroids. 59: 514.
11. Ide H, et al. 2002. Proc Natl Acad Sci USA. 99: 14404.
12. Ohno H, et al. 2006. Mol Cancer Ther. 5: 2634.
13. Rovida E, et al. 2001. J Immunol. 166: 1583.
14. Wrobel CN, et al. 2004. J Cell Biol. 165: 263.
15. Cannarile MA, et al. 2017. J Immunother Cancer. 5: 53.
16. Guo J, et al. 2006. Mol Cancer Ther. 5: 1007.
17. Hiraga T, et al. 2009. Int J Cancer. 124: 215.
18. Machkani B, et al. 2010. Bioorg Med Chem. 18: 1789.
19. Battisti C, et al. 2014. J Neurol. 261: 768.

Gene ID
12978 View all products for this Gene ID
UniProt
View information about CSF-1R on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
Go To Top Version: 1    Revision Date: 09.09.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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