Recombinant Mouse B7-H2 (ICOSL)-Fc Chimera (carrier-free)

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Other Names
Inducible T Cell Costimulator Ligand, B7-Related Protein, B7 Homolog 2, ICOS Ligand, ICOSL, B7RP-1, B7-H2, B7H2, ICOS-L, CD275, GL50, B7h
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Mouse_B7-H2_ICOSL_CF_RECOM_1_111020
When recombinant mouse B7-H2 (ICOSL)-Fc chimera is immobilized at 2 µg/mL, biotinylated mouse ICOS binds in a dose-dependent manner. The EC50 range for this effect is 50 - 200 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Mouse_B7-H2_ICOSL_CF_RECOM_1_111020
    When recombinant mouse B7-H2 (ICOSL)-Fc chimera is immobilized at 2 µg/mL, biotinylated mouse ICOS binds in a dose-dependent manner. The EC50 range for this effect is 50 - 200 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Mouse_B7-H2_ICOSL_CF_RECOM_2_111020
    Stability Testing for Recombinant Mouse B7-H2 (ICOSL)-Fc Chimera. Recombinant mouse B7-H2 (ICOSL)-Fc chimera was aliquoted in PBS at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in a binding assay with biotinylated mouse ICOS.
Cat # Size Price Quantity Avail. Save
792704 25 µg 96€
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792706 100 µg 248€
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Description

B7-H2 belongs to the B7 family of immunoregulatory molecules and is a transmembrane glycoprotein of approximately 60 kD. Mature mouse B7-H2 consists of a 231 amino acid extracellular domain (ECD), two immunoglobulin-like domains, a 21 amino acid transmembrane segment, and a 24 residue cytoplasmic domain. Within the ECD, human B7-H2 shares 50% and 54% amino acid sequence identity with mouse and rat B7-H2, respectively. B7-H2 is expressed on antigen presenting cells such as B cells, macrophages, dendritic cells, and also in monocytes. It binds to ICOS and is expressed on activated T cells, which leads to both positive and negative effects on immune responses including its own downregulation. Mouse and human B7-H2 exhibit cross species binding to ICOS. B7-H2 interaction with ICOS is costimulatory for T cell proliferation as well as the development of B cells, plasma cells, follicular helper T cells, and germinal centers. In addition, human B7-H2 binds to CD28 and CTLA4.  The interaction of human B7-H2 and CD28 can costimulate both human mouse naïve T cells and regulatory T cells (Treg). B7-H2 plays a role in the development of allergic asthma by enhancing Th2 biased immune responses, limiting Th17 responses, and promoting eosinophilic infiltration into the lungs. B7-H2 activation of ICOS on Tregs limits pulmonary inflammation and airway hyper-responsiveness, promotes the development of inhalational tolerance, and impairs anti-tumor immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse B7-H2, amino acid Glu47-Lys279 (Accession # Q9JHJ8), with a C-terminal mouse IgG2a (Glu99-Lys330), was expressed in CHO cells.
Molecular Mass
The 473 amino acid recombinant protein has a predicted molecular mass of approximately 53.5 kD. The DTT-reduced and non-reduced proteins migrate at approximately 80 kD and 160 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Glu.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
When recombinant mouse B7-H2 (ICOSL)-Fc chimera is immobilized at 2 μg/mL, biotinylated mouse ICOS binds in a dose-dependent manner. The EC50 range for this effect is 50 - 200 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Disulfide linked homodimer
Distribution

B cells, macrophages, monocytes, and dendritic cells

Function
The B7-H2/ICOS signal induces T-cell proliferation and secretion of both Th1 and Th2 cytokines, such as IL-4 and IFN-γ, but not IL-2 (it also induces IL-5 and TNF-α production). B7-H2/ICOS signal may favor the induction of Th2-type responses and thus may be involved in the negative regulation of cell-mediated immune responses against tumors. B7-H2 is induced by TNF-α in B cells and monocytes and down-regulated in dendritic cells.
Interaction
Activated T cells
Ligand/Receptor
ICOS
Bioactivity
Measured by its ability to bind recombinant mouse ICOS
Cell Type
Dendritic cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction
Molecular Family
Soluble Receptors, TCRs
Antigen References

1. Ishikawa K, et al. 1998. DNA Res. 5:169.
2. Yoshinaga SK, et al. 2000. Int. Immunol. 12:1439.
3. Flesch IE, 2003. J. Biol. Regul. Homeost. Agents. 16:217.
4. Akbari O, et al. 2002. Nat. Med. 8:1024.
5. Yao S, et al. 2011. Immunity. 34:729.

Gene ID
50723 View all products for this Gene ID
UniProt
View information about B7-H2 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 09.17.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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