Recombinant Human sTNF-RII (TNFRSF1B) (carrier-free)

Pricing & Availability
Other Names
p75TNFR, cd120b, TNF RII, tumor necrosis factor receptor superfamily member 1B (TNFRSF1B), TNFBR, TNFR80, TNF receptor beta chain, p75 TNFR, p80 TNF-alpha receptor, tumor necrosis factor receptor 2.
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Product Citations
publications
sTNFRII_Human_Recombinant_BA_102913
Inhibition of TNFα-induced cytotoxicity in L929 cells by soluble TNF-RII.
  • sTNFRII_Human_Recombinant_BA_102913
    Inhibition of TNFα-induced cytotoxicity in L929 cells by soluble TNF-RII.
Cat # Size Price Quantity Avail. Save
591608 500 µg 1 268€
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591602 10 µg 100€
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591604 25 µg 172€
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591606 100 µg 468€
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Cat. No. 591608 is scheduled for discontinuation. Please use the availability checker to determine current availability. The smaller sizes are available as an alternative.
Description

The biological effects of TNF are mediated by two cell surface TNF receptors, TNF-RI and TNF-RII. TNF-RI and TNF-RII are structurally related but functionally distinct. Also, TNF-RI is widely expressed on most cells, whereas TNF-RII is more restricted and is primarily expressed in hematopoietic cells and cells of the immune system. The function of TNF-RII is less understood and has fewer biological effects than TNF-RI. Unlike TNF-RI, TNF-RII does not have death domain and will induce NF-κB activation through a different pathway. It is generally accepted that TNF-RI induces both apoptosis and survival pathways and TNF-RII induces only survival pathway. Only membrane-bound TNF can active TNF-RII effectively. Soluble TNF can bind TNF-RII but can not induce TNF-RII signaling. Upon binding of TNF, TNF-RII will form a trimer and recruit TRAF2, therefore leading to activation of NF-κB. In T cells, TNF-RII costimulation provides survival signals during the differentiation program trigger by TCR-mediated stimulation and also during clonal expansion in response to intracellular bacterial pathogens. Both TNF-RI and TNF-RII can be released from cell surface by ectodomain shedding. The resulting soluble TNF-RI and TNF-RII can bind TNF with high affinity and modulate TNF effects. An in vitro study showed that both TNF-RI and TNF-RII are constitutively released from monocytes. LPS stimulation can further increase soluble TNF-RII release. It has been reported that association of TNF-RII polymorphism with some lymphomas, solid tumors, and autoimmune diseases. The plasma level of soluble TNF-RII is increased in obese people and is related to insulin resistance. In addition, recombinant Fc-tagged soluble TNF-RII has been used to treat rheumatoid arthritis.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human TNF-RII, amino acids Pro 24-Thr206 with an N-terminal Met (Accession# AAA59929.1) was expressed in E. coli.
Molecular Mass
The 184 amino acid recombinant protein has a predicted molecular mass of approximately 20 kD. The DTT-reduced protein migrates at approximately 21 kD and the non-reduced protein migrates at approximately 19 kD by SDS-PAGE. The N-terminal amino acid is Met.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 is 0.15-0.5 µg/ml, corresponding to a specific activity of 2-6 x 103 units/mg, as determined by a dose-dependent inhibition of 0.25 ng/ml TNF-α induced cytotoxicity in L929 mouse fibroblast cells in the presence of 4 µg/ml actinomycin D.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Cytokine receptor
Distribution

TNF-RII is expressed in T cell, astrocytes, oligodendrocytes, monocytes, endothelial cells, human mesenchymal stem cells, macrophages and Langerhans cells. Soluble TNF-RII can be detected in the urine and blood.

Function
TNF-RII transduce TNF signal through TRAF2. Soluble TNF-RII can neutralize the biological activity of TNF, dampening the inflammatory response. The level of circulating soluble TNF-RII is increased in patients with sepsis, cancer, HIV, and autoimmune disease.
Ligand/Receptor
TNF
Cell Type
Dendritic cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Mitochondrial Function
Molecular Family
Cytokine/Chemokine Receptors, Soluble Receptors
Antigen References

1. Hehlgans T and Mannel DN. 2002. Biol. Chem. 383:1581.
2. Glossop, et al. 2005. Arthritis. Res. Ther. 7:R1227.
3. Kollias G and Kontoyiannis D. 2002. Cytokine Growth Factor Rev. 13:135.
4. Gordon AC, et al. 2004. Genes Immun. 5:631.
5. Lantz M et al. 1990. Cytokine 2:402.
6. Nophar Y et al. 1990. EMBO. 9:3269.
7. Naude PJ et al. 2011. FEBS J. 278:888.

Gene ID
7133 View all products for this Gene ID
UniProt
View information about CD120b on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 3    Revision Date: 10.17.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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