Recombinant Human IL-4 (293E-expressed) (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
B cell growth factor 1 (BCGF-1), B-cell stimulatory factor 1 (BSF-1), interleukin-4, lymphocyte stimulatory factor 1.
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Product Citations
publications
Human_IL-4_293E-expressed_RECOM_CF_071717
Human IL-4 induces proliferation of TF 1 human erythroleukemic cells.
  • Human_IL-4_293E-expressed_RECOM_CF_071717
    Human IL-4 induces proliferation of TF 1 human erythroleukemic cells.
Cat # Size Price Quantity Avail. Save
766208 500 µg 1552,00€
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766202 10 µg 68,00€
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766204 25 µg 160,00€
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766206 100 µg 512,00€
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Description

IL-4 is the primary cytokine implicated in the development of Th2-mediated responses, which is associated with allergy and asthma. IL-4 binds to two receptor complexes, the Type I receptor comprises IL-4Rα and the common gamma-chain (γc), which is also shared by the cytokines IL-2, -7, -9, -15 and -21 and is present in hematopoietic cells. IL-4 can use the type II complex, comprising IL-4Rα and IL-13Rα1, which is present in non-hematopoietic cells. This second receptor complex is a functional receptor for IL-13, which shares approximately 25% homology with IL-4. The type I receptor complex can be formed only by IL-4 and is active in Th2 development. In contrast, the type II receptor complex formed by either IL-4 or IL-13 is more active during airway hypersensitivity and mucus secretion and is not found in T cells.IL-4, through its activation of STAT6, upregulates GATA3 expression and also suppresses TH1 and TH17 cell responses, partly through the upregulation of growth factor independent 1(GFI1), a transcriptional repressor of IFNγ and IL-17 production. IL-4 induces macrophage activation and TSLP production. IL-4 recruits and activates IgE-producing B cells (IgE class switching) and enhances IgE-mediated responses by up-regulating IgE receptors on B lymphocytes, mast cells, and basophils. In addition, IL-4 also induces VCAM-1 on vascular endothelium and thus directs the migration of T lymphocytes, monocytes, basophils, and eosinophils to the inflammation site.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human IL-4, amino acids (His25 – Ser153) (Accession #NM_000589) was expressed in 293E cells.
Molecular Mass
The 129 amino acid recombinant protein has a predicted molecular mass of approximately 14.9 kD. The DTT-reduced and non-reduced protein migrate at approximately 20 kD and 18 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is His.
Purity
> 95% by SDS-PAGE gel as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 0.1 EU per µg (0.01 ng/µg) cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Human IL-4 induces proliferation of TF 1 human erythroleukemic cells in a dose dependent manner. The ED50 = 0.04 - 0.2 ng/ml, corresponding to a specific activity of 0.5 -2.5 x 107 units/mg.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Product Citations
  1. Eken A, et al. 2020. North Clin Istanb. 0.513194444. PubMed

Antigen Details

Structure
Heterodimer
Distribution

IL-4 is produced by Th2 cells, naive CD4<sup>+</sup> T cells, NKT cells, and basophils.

Function
Major role in differentiation of TH2 cells and induction of Th2 associated cytokines. Suppresses TH1 and TH17 cell responses, induces macrophage activation and TSLP production, enhances IgE-mediated in B lymphocytes, mast cells, and basophils.
Interaction
T cells, B cells, macrophages, epithelial cells, smooth muscle cells, and bronchial fibroblasts.
Ligand/Receptor
IL-4 signals through Type I (IL-4Rα, γc) and Type II receptors (IL-4Rα, IL-13Rα1) complexes.
Cell Type
Hematopoietic stem and progenitors, Embryonic Stem Cells
Biology Area
Stem Cells, Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Swain SL, et al. 1990. J. Immunol. 145:3796.
2. Hsieh CS, et al. 1992. Proc. Natl. Acad. Sci. USA 89:6065.
3. Allison-Lynn A, et al. 2006. J. Immunol. 176:7456.
4. Kato A, et al. 2007. J. Immunol. 179:1080.
5. LaPorte SL, et al. 2008. Cell 132:259.
6. Martinez FO, et al. 2009. Annu. Rev. Immunol. 27:451.
7. Luzina IG, et al. 2012. J. Leuko. Biol. 92:753.

Gene ID
3565 View all products for this Gene ID
UniProt
View information about IL-4 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
Go To Top Version: 0    Revision Date: 07.19.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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