Recombinant Human CD27 (TNFRSF7)-Fc Chimera (carrier-free)

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Other Names
CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7
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Human_CD27_TNFRSF7_RECOM_CF_071317
Immobilized recombinant human CD27 at 2 µg/ml binds recombinant human CD27L with an ED50 = 8 - 40 ng/ml.
  • Human_CD27_TNFRSF7_RECOM_CF_071317
    Immobilized recombinant human CD27 at 2 µg/ml binds recombinant human CD27L with an ED50 = 8 - 40 ng/ml.
Cat # Size Price Quantity Avail. Save
766002 10 µg 84€
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766004 25 µg 128€
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Description

CD27 (TNFRSF7) is a member of the TNFR family. It is a type I membrane glycoprotein with a MW of 55KDa that forms a disulfide‑linked homodimer. The receptor possesses three cysteine rich repeats in the extracellular domain, the transmembrane region, and the intracellular domain. CD27 binds CD27L (CD70), another member of the TNF ligand family that forms a trimer; thus, it has been speculated that the CD27L binds to a trimeric structure composed by three dimeric CD27 molecules. The interaction between CD27 and its ligand, CD70, plays a role in providing co-stimulation for prolonged lymphocyte survival, enhanced T-cell proliferation, and memory-cell formation. T cells expressing CD27 and CD70 play a key role in regulating B-cell activation and immunoglobulin synthesis. Soluble CD27 (sCD27), the extracellular domain of membrane-bound CD27, can be released after lymphocyte activation by differential splicing of the receptor protein or shedding from the cell surface by metalloproteinases (MMPs). Soluble CD27 has been detected in serum, plasma, and urine samples from healthy individuals, and it has been described elevated sCD27 levels in serum of patients with systemic lupus erythematosus, viral infections, and lymphoid malignancies. The sCD27-pool in cancer patients can be significantly enlarged by immunotherapy. CD27 is recognized as a marker for memory B cells and is considered of diagnostic value in patients who have decreased numbers of switched memory B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human CD27, amino acids (Thr21-Ile192) (Accession # NP_001233) was expressed in 293E cells. The carboxy terminus contains SRIEGRMD-hIgG-Fc tag.
Molecular Mass
The 411 amino acid recombinant protein has a predicted molecular mass of approximately 46.1 kD. The DTT-reduced and non-reduced protein migrate at approximately 60 kD and 120 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Thr.
Purity
>95% by SDS-PAGE gel as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS pH 7.2.
Endotoxin Level
Less than 0.01 EU per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Measured by functional ELISA. Immobilized human recombinant CD27 at 2 µg/ml binds human CD27L (BL cat.# 553404) with an ED50 = 8 - 40 ng/ml.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Homodimer
Distribution

Expressed on resting T and B cells, NK cells, and hematopoietic progenitor cells.

Function
Induces T-cell costimulation and B-cell activation, plasma cell differentiation.
Interaction
Activated T and B cells, APC
Ligand/Receptor
CD27L (CD70)
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, Immune Checkpoint Receptors, Soluble Receptors
Antigen References

1. Camerini D, et al. 1991 J. Immunol. 147:3165.
2. Prasad KV, et al. 1997. Proc. Natl. Acad. Sci. 94:6346.
3. Kato K, et al. 2007 Exp. Hematology 35:434.
4. van Montfrans J M, et al. 2012. J. Allergy Clin. Immun. 129: 787.
5. Goto N, et al. 2012. Leuk. Lymphoma. 53:1494.
6. Huang J, et al., 2013. J. Immunol. 190:6250. 

Gene ID
939 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 0    Revision Date: 07.21.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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