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Purified Streptavidin

Pricing & Availability
Regulatory Status
RUO
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Product Citations
publications
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280302 250 µg 34€
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Description

Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used in a wide range of applications including ELISA, flow cytometry, molecular biology, and bionanotechnology.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
Phosphate-buffered solution containing 0.09% sodium azide.
Concentration
1.0 mg/ml
Storage & Handling
Store between 2°C and 8°C.
Application

FC - Quality tested
ELISA Capture, ELISA Detection, ICC, IHC-F, IHC-P, IP, WB - Reported in the literature, not verified in house
Recommended Usage

To coat one 96-well plate dilute 24 µL of Purified Streptavidin into 11.976ml of 1X Coating Buffer (Cat. No. 421701, Coating Buffer 5X concentrate). Final concentration in the coated plate is 2 µg/mL. This recommendation is to perform the protocol for HLA class I ELISA to evaluate Flex-T™ peptide exchange. For other applications the reagent needs to be optimized.

Product Citations
  1. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
  2. Wu C, et al. 2020. J Am Chem Soc. 142:12314. PubMed

Antigen Details

Structure
52.8 kD tetrameric protein.
Distribution

Streptavidin binds to biotin with high affinity.

Ligand/Receptor
Biotin.
Antigen References

1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343.
2. Zhao X, et al. 2013. J. Anal. Methods Chem. 2013:581093.
3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135.
4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113.
5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.

Gene ID
NA

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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