Purified Mouse IgG2b, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MPC-11 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
MPC-11_ChIP_IgG2b-k_ctrl_1_060618_updated.png
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400375) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • MPC-11_ChIP_IgG2b-k_ctrl_1_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400375) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • MPC-11_ChIP_IgG2b-k_ctrl_2_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400375) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MDM2 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
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400301 50 µg 20,00€
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400302 500 µg 80,00€
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Description

The MPC-11 immunoglobulin has unknown specificity. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The immunoglobulin solution should be stored undiluted between 2°C and 8°C
Application

FC - Quality tested
ChIP - Verified
ICFC, ICC, IHC, IP, WB, FA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).

Application References

(PubMed link indicates BioLegend citation)
  1. Smed-Sörensen A, et al. 2008. Blood 111:5037. (FA) PubMed
  2. Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC) PubMed
Product Citations
  1. Tognarelli S, et al. 2018. Front Immunol. 9:2743. PubMed
  2. Hartley GP, et al. 2018. Cancer Immunol Res. 6:1260. PubMed
  3. Zhong Q, et al. 2018. J Immunol. 200:3913. PubMed
  4. Mastoridis S, et al. 2018. Front Immunol. 1.474305556. PubMed
  5. Hu X, et al. 2020. Neoplasia. 1.290972222. PubMed
  6. Smed-Sörensen A, et al. 2008. Blood. 111:5037. PubMed

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 08.24.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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