Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P)

Pricing & Availability
Clone
Poly18020 (See other available formats)
Regulatory Status
RUO
Other Names
CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, TUBB3, tubulin beta-4 chain, class III beta-tubulin
Previously
Covance Catalog# PRB-435P
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
A_Poly18020_PURE_Tubulin_beta3_IHCP_1_032918
IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded rat cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A_Poly18020_PURE_Tubulin_beta3_IHCP_1_032918
    IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded rat cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • B_Poly18020_PURE_Tubulin_beta3_IHCP_2_032918
    IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded human cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • C_Poly18020_PURE_Tubulin_beta3_IHCP_3_032918
    IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded human cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly18020_PURE_Tubulin_beta3_WB_021915.jpg
    Western blot of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 0.1 µg/mL of Poly18020 or Rabbit IgG overnight at 4°C, followed by incubation with HRP-labeled anti-rabbit IgG (Cat. No. 410406). Enhanced chemiluminescence was used as the detection system.
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802001 100 µL 347€
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Description

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antibody was raised against microtubules derived from rat brain.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
ICC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 0.5 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunocytochemistry (ICC).

This antibody is well characterized and highly reactive to neuron specific Class III ß-Tubulin (ßIII), but does not identify ß-tubulin found in glial cells. This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Feuer R, et al. 2005. J. Neurosci. 25(9):2434-44.
  2. Mozzetti S, et al. 2005. Clin. Cancer Res. 11(1):298-305.
  3. Feuer R, et al. 2003. Am. J. Pathol. 163(4):1379-93.
  4. Zhang XM, Yang XJ. 2001. Development. 128(6):943-57.
  5. Ma M, et al. 2012. Neurobiol Dis. 56:34. (WB) PubMed
  6. Tseung G, et al. 2011. J. Virol. 85:5718. (IF) PubMed
  7. Shimada IS, et al. 2012. J Neurosci. 32:7926. (IHC) PubMed
  8. Flores-Otero J, et al. 2007. J. Neurosci. 27:14023. (IF) PubMed
Product Citations
  1. Tseng K, et al. 2017. eNeuro. 10.1523/ENEURO.0214-17.2017. PubMed
  2. Ahangari N, et al. 2021. J Neuropathol Exp Neurol. 80:944. PubMed
  3. Samarasinghe RA, et al. 2021. Nat Neurosci. 24:1488. PubMed
  4. Chow A, Morshead C 2016. Stem Cell Res. 16:79-87. PubMed
  5. Son M, et al. 2016. Exp Mol Med. 48: 232. PubMed
  6. Hoshino N, et al. 2021. Journal of Comparative Neurology. :. PubMed
  7. Arber C, et al. 2019. Brain Commun. 1:fcz024. PubMed
  8. Jiang Y, et al. 2018. J Biol Chem. 293:19317. PubMed
  9. Stokowska A, et al. 2017. Brain. 140:353. PubMed
  10. Eltahir MK, et al. 2022. PLoS One. 17:e0269683. PubMed
  11. Pardieck J, et al. 2021. Stem Cells Dev. 30:816. PubMed
  12. Kim S, et al. 2021. Nat Commun. 12:1807. PubMed
  13. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  14. Tian E, et al. 2016. Cell Rep. 16: 781-792. PubMed
  15. Baleriola J, et al. 2016. Sci Rep. 6: 25928. PubMed
  16. Shimada I, et al. 2012. J Neurosci. 32:7926-7940. PubMed
  17. Brosig A, et al. 2019. Cell Rep. 29:2028. PubMed
  18. Girouard MP, et al. 2020. eneuro. 7(2):ENEURO. PubMed
  19. Meng QT, et al. 2021. Elife. 10:. PubMed
  20. Olmsted ZT, et al. 2022. Front Cell Neurosci. 15:725195. PubMed
  21. Binder J, et al. 2022. Commun Biol. 5:125. PubMed
  22. Krishnan V, et al. 2016. J Neuropathol Exp Neurol. 75: 464 - 478. PubMed
  23. Vogt M, et al. 2021. Theranostics. 11:5569. PubMed
  24. Pires G, et al. 2021. Brain Commun. 3:fcab021. PubMed
  25. Nichterwitz S, et al. 2020. Genome Res. 30:1083. PubMed
  26. Xie Y, et al. 2018. Stem Cell Res. 9:2010. PubMed
  27. Uezono N, et al. 2018. Stem Cells. 36:737. PubMed
  28. Tang SC, et al. 2017. Diabetologia. 61:158. PubMed
  29. MOSES C, et al. 2020. Sci Rep. 10:11393. PubMed
  30. Nayak G, et al. 2020. Cell Reports. 30(3):672-686.e8.. PubMed
  31. Brykczynska U, et al. 2016. Stem Cell Reports. 7:1059-1071. PubMed
  32. Omichi R, et al. 2020. Mol Ther Methods Clin Dev. 1.51875. PubMed
  33. Hastings RL, et al. 2020. Sci Rep. 10:11132. PubMed
  34. Hoffman LF, et al. 2018. J Assoc Res Otolaryngol. 19:33. PubMed
  35. Carlin D et al. 2018. eNeuro. 5(2) pii: ENEURO. PubMed
  36. Dvoriantchikova G, et al. 2022. Sci Rep. 12:17152. PubMed
  37. Jo S, et al. 2021. Stem Cell Research. 56:102524. PubMed
  38. Gong C, et al. 2021. Cell Reports. 34(12):108889. PubMed
  39. Kurihara Y, et al. 2020. Journal of Neurochemistry. 155(3):285-299. PubMed
  40. Sakaguchi H, et al. 2019. Stem Cell Reports. 13:458. PubMed
  41. Voronova A et al. 2017. Neuron. 94(3):500-516 . PubMed
  42. Sabelström H, et al. 2019. Cell Rep. 28:2064. PubMed
  43. Mertens J, et al. 2021. Cell Stem Cell. 28:1533. PubMed
  44. Boland M, et al. 2017. Brain. 10.1093/brain/aww357. PubMed
  45. Ou MY, et al. 2021. Cell Reports. 35(13):109290. PubMed
  46. Verstraelen P, et al. 2017. Front Cell Neurosci. 11:173. PubMed
  47. Kim H et al. 2017. Stem cell research. 26:72-75 . PubMed
  48. Li H, et al. 2019. iScience. 19:559. PubMed
  49. van Niekerk EA, et al. 2022. Cell Rep Methods. 2:100255. PubMed
  50. Avraham O, et al. 2021. Elife. 10:. PubMed
  51. Simone R, et al. 2021. Nature. 594:117. PubMed
  52. Lasagni Vitar RM, et al. 2021. Invest Ophthalmol Vis Sci. 62:26. PubMed
  53. Reddy A, et al. 2020. Cell. 183:62. PubMed
  54. Otani T, et al. 2016. Cell Stem Cell. 18: 467-480. PubMed
  55. Benoy V, et al. 2018. Brain. 10.1093/brain/awx375. PubMed
  56. Chabrat A, et al. 2019. Stem Cells Int. 2019:2945435. PubMed
  57. Yang C, et al. 2020. Neuron. 105:276. PubMed
  58. Suter TACS, et al. 2020. Cell Rep. 30:1164. PubMed
  59. Macpherson P, et al. 2015. Development. 142: 4038 - 4048. PubMed
  60. Ferguson M, et al. 2013. Neurobiol Dis. 56:34-46. PubMed
  61. Yau K, et al. 2016. J Neurosci. 36: 1071 - 1085. PubMed
  62. Cui Q, et al. 2016. Nat Commun. 7:10637. PubMed
  63. Bosze B, et al. 2020. J Neurosci. 40:1501. PubMed
  64. Iannielli A, et al. 2019. Cell Rep. 29:4646. PubMed
  65. Bowles KR, et al. 2019. PLoS One. 14:e0213374. PubMed
  66. Toda T, et al. 2017. Cell Stem Cell. 21:618. PubMed
  67. Adams MT, et al. 2021. eLife. 0.416666666666667. PubMed
  68. Qiang L et al. 2018. Current biology : CB. 28(13):2181-2189 . PubMed
  69. Meijer M et al. 2019. Cell Rep. 27(7):2199-2211 . PubMed
  70. Rhee HJ et al. 2019. Cell Rep. 27(7):2212-2228 . PubMed
  71. Makar T, et al. 2018. Stem Cell Res Ther. 9:127. PubMed
  72. Zahr SK et al. 2018. Neuron. 97(3):520-537 . PubMed
  73. Amador A, et al. 2020. Brain. 143:2039. PubMed
  74. Finno CJ, et al. 2022. Front Cell Neurosci. 16:853035. PubMed
  75. Newland B, et al. 2021. ACS Chem Neurosci. 12:1178. PubMed
  76. Sugiyama T, et al. 2021. iScience. 24(7):102758. PubMed
  77. Bhaskaran S, et al. 2020. Aging Cell. 19:e13225. PubMed
  78. Cora V, et al. 2019. Cells. 8:5. PubMed
  79. Grove M, et al. 2020. eLife. 9:e50138.. PubMed
  80. Alvarez–Lindo N, et al. 2019. Sci Rep. 9:18486. PubMed
  81. Tiwari D et al. 2019. Neurobiol Dis. 1871:48:00 . PubMed
  82. Carlin D et al. 2019. eNeuro. 6(3) pii: ENEURO. PubMed
  83. Li H, et al. 2018. Hum Mutat. 39:550. PubMed
  84. Francistiová L, et al. 2022. Front Mol Neurosci. 14:793769. PubMed
  85. Iennaco R, et al. 2022. Cell Death Differ. 29:293. PubMed
  86. Bjorke B, et al. 2021. Dev Biol. 476:272. PubMed
  87. Huang S, et al. 2020. Cell. 184(2):441-459.e25. PubMed
  88. Lin P, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G412 - G422. PubMed
  89. Tsueng G, et al. 2011. J Virol. 85:5718-5732. PubMed
  90. Ou J et al. 2018. Cell. 173(4):851-863 . PubMed
  91. Hsu J, et al. 2019. Nat Commun. 10:2939. PubMed
  92. Chanda S et al. 2019. Cell Stem Cell. 25(1):103-119 . PubMed
  93. Peitz M, et al. 2020. STAR Protoc. 1:100038. PubMed
  94. Smith LA, et al. 2021. Front Cell Dev Biol. 9:667246. PubMed
  95. Mao Y, et al. 2021. Cell Prolif. 54:e13090. PubMed
  96. Olmsted ZT, et al. 2022. iScience. 25:104486. PubMed
  97. Vahsen BF, et al. 2022. Sci Rep. 12:12606. PubMed
  98. Watanabe S, et al. 2016. EMBO Mol Med. 8(12):1421-1437. PubMed
  99. Cates K, et al. 2020. Cell Stem Cell. 28(1):127-140.e9. PubMed
  100. Barry DM, et al. 2020. Nat Commun. 11:1397. PubMed
  101. Hattori Y, et al. 2020. Nat Commun. 11:1631. PubMed
  102. Duncan DS, et al. 2018. Front Neurosci. 0.9875. PubMed
  103. Martínez JC et al. 2019. Neuron. 104(5):931-946 . PubMed
  104. Mickle AD, et al. 2019. Nature. 565:361. PubMed
  105. Kawakami Y et al. 2018. The Journal of Neuroscience. 38(10):2589-2604 . PubMed
  106. Flitsch LJ, et al. 2022. Sci Rep. 12:8356. PubMed
  107. Pahl MC, et al. 2021. Nat Commun. 12:6749. PubMed
  108. Kim H, et al. 2020. Sci Signal. 13: . PubMed
  109. Vicidomini C, et al. 2016. Mol Psychiatry. 10.1038/mp.2016.30. PubMed
  110. Shah K, et al. 2019. J Mol Cell Biol. N/A. PubMed
  111. Sar Shalom H, et al. 2019. Elife. 8:e41162. PubMed
  112. Facchin C, et al. 2020. Theranostics. 10:3518. PubMed
  113. Flores-Otero J, et al. 2007. J Neurosci. 27:14023-14034. PubMed
  114. Zhang L, et al. 2016. Neuroscience. 337:88-97. PubMed
  115. Arber C, et al. 2021. Cell Reports. 34(2):108615. PubMed
  116. Shang Y, et al. 2018. J Neurosci. 38:4006. PubMed
  117. Jean-Louis T, et al. 2018. Neurobiol Aging. 62:130. PubMed
  118. Baker L, et al. 2021. JCI Insight. 6:. PubMed
  119. Besusso D, et al. 2020. Stem Cell Res. 102016:49. PubMed
  120. Hung COY et al. 2018. Cell reports. 25(13):3647-3660 . PubMed
  121. Jeon H, et al. 2018. J Clin Med. 7:. PubMed
  122. Dan W, et al. 2022. Front Physiol. 12:744294. PubMed
  123. Avraham O, et al. 2022. Front Mol Neurosci. 14:796070. PubMed
  124. Jacob L, et al. 2022. J Exp Med. 219:. PubMed
  125. Arber C, et al. 2020. Mol Psychiatry. 25:2919. PubMed
  126. Smith-Anttila CJA, et al. 2021. Developmental Biology. 476:18-32. PubMed
  127. Hung C, et al. 2021. Cell Reports. 35(11):109259. PubMed
  128. Ornaghi F, et al. 2020. Neurobiol Dis. 134:104667. PubMed
  129. Lee J, et al. 2018. J Clin Med. 7:. PubMed
  130. Avraham O, et al. 2022. Front Mol Neurosci. 15:967472. PubMed
  131. Su Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  132. Olmsted ZT, et al. 2021. iScience. 24(8):102827. PubMed
  133. Ward AI, et al. 2020. MBio. 11:00. PubMed
  134. Avraham O, et al. 2020. Nat Commun. 3.854861111. PubMed
  135. Anastasiadou S, Knöll B 2016. Exp Neurol. 279:243-260. PubMed
RRID
AB_2564645 (BioLegend Cat. No. 802001)

Antigen Details

Cell Type
Mature Neurons, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Microtubules
Gene ID
10381 View all products for this Gene ID
UniProt
View information about Tubulin beta-3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 10.24.2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-Tubulin β-3 (TUBB3)

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