Purified anti-Tollip Antibody

Pricing & Availability
Clone
O92A4 (See other available formats)
Regulatory Status
RUO
Other Names
Tollip, Toll Interacting Protein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
O92A4_Pure_Tollip_Antibody_1_082718
Western blot of purified anti-Tollip antibody (clone O9I2A4). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • O92A4_Pure_Tollip_Antibody_1_082718
    Western blot of purified anti-Tollip antibody (clone O9I2A4). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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854401 25 µg 72,00€
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854402 100 µg 188,00€
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Description

Tollip, also known as Toll interacting protein, is a ubiquitin-binding protein and an inhibitory adaptor protein involved  in IL-1 and Toll-like receptor (TLR) signaling pathway.  Alterations in Tollip gene expression levels has been linked to  tuberculosis and idiopathic pulmonary fibrosis, suggesting a role for Tollip in innate immune system. Tollip has also been reported to mediate autophagic clearance of poly-ubiquitinated proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human recombinant Tollip (NP_061882) produced in HEK293T cell.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is highly specific to human Tollip. It does not cross react with murine Tollip.

RRID
AB_2750411 (BioLegend Cat. No. 854401)
AB_2750472 (BioLegend Cat. No. 854402)

Antigen Details

Structure
Tollip is a 274 amino acid protein with an apparent molecular mass of ~30 kD.
Distribution

Tissue Distribution: Ubiquitously expressed.
Cellular Distribution: Plasma membrane, extracellular, cytosol, lysosome, and nucleus.

Function
Tollip is a component of the IL-1 and TLR signaling pathway involved in innate immune response.
Interaction
Tollip has been reported to interact with TOM1, TLR2, TLR4, IRAK1, IR-1RI, TOM1L2, and ILRAP.
Biology Area
Cell Biology, Immunology, Innate Immunity, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Enzymes and Regulators
Antigen References
  1. Capelluto DG. 2012. Microbes Infect. 14(2): 140
  2. Brandao I, et al. 2015. Gut Microbes. 6(3):202
Gene ID
54472 View all products for this Gene ID
UniProt
View information about Tollip on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08.27.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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