Purified anti-Synaptobrevin Antibody (Previously Covance catalog# MMS-636P)

Pricing & Availability
Clone
SP11 (See other available formats)
Other Names
Vesicle-associated membrane protein 1, synaptobrevin-1, VAMP-1, SYB1
Previously
Covance Catalog# MMS-636P
Isotype
Mouse IgG1
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Product Citations
publications
1_SP11_PURE_Synaptobrevin_Antibody_WB_051418
Western blot of purified anti-Synaptobrevin antibody (clone SP11). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 2 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • 1_SP11_PURE_Synaptobrevin_Antibody_WB_051418
    Western blot of purified anti-Synaptobrevin antibody (clone SP11). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 2 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • 2_SP11_PURE_Synaptobrevin_Antibody_ICC_051418
    ICC staining of purified anti-Synaptobrevin antibody (clone SP11) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 1 µg/ml of the primary antibody for overnight at 4°C, followed by incubation wit 5 µg/ml of Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLongTM Gold Antifade Mountant. The image was captured with a 60X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
805101 100 µL 230€
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This product is scheduled for discontinuation. Please use the availability checker to determine current availability. Cat. Nos. 837003 and 837004 are available as alternatives.
Description

Synaptobrevins, also known as VAMPs, along with syntaxins and the synaptosomal-associated protein SNAP25, form the protein complex that is involved with the docking and fusion of synaptic vesicles with the presynaptic membrane. Synaptobrevins have a molecular weight of 18 kD, and are also involved in the formation of the SNARE complexes. The synaptobrevins are degraded by the protein tetanospasmin, from the tetanus-causing bacterium Clostridium tetani, as well as botulinum toxin from Clostridium botulinum.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested

ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 10 µg per ml. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. 

Application Notes

This antibody is effective in immunoblotting (WB), immunocytochemistry (ICC).

SP11 reacts with native, denatured and recombinant synaptic vesicle proteins synaptobrevin/VAMP-1 (aa 1-118).  

Application References

(PubMed link indicates BioLegend citation)
  1. Tsuboi Y, et al. 2003. Neurology. 60:1766.
  2. Masliah E, et al. 1994. Acta Neuropath. 87:135.
  3. Mallory M, et al. 1999. Acta Neuropath. 97:437.
  4. Honer WG, et al. 1993. Brain Res. 609:9.
RRID
AB_2564675 (BioLegend Cat. No. 805101)

Antigen Details

Structure
Expected MW: 16 kD
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Gene ID
6843 View all products for this Gene ID
UniProt
View information about Synaptobrevin on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 03.19.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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