Purified anti-Strep-Tag Antibody

Pricing & Availability
Clone
BL26882 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
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Product Citations
publications
BL26882_PURE_Strep-tag_Antibody_1_WB_092216
Recombinant Strep tag fused human Her2 (23-652 aa) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti- Strep-Tag (clone BL26882) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • BL26882_PURE_Strep-tag_Antibody_1_WB_092216
    Recombinant Strep tag fused human Her2 (23-652 aa) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti- Strep-Tag (clone BL26882) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
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688202 25 µg 60,00€
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Description

The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Strep-Tag fusion proteins
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic Peptide
Formulation
PBS pH: 7.4, containing 0.5 % BSA, 0.02% sodium azide, and 50% glycerol.
Preparation
The antibody was affinity purified from mouse ascites by affinity chromatography using specific immunogen.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Xu Z, et al. 2021. Cell Death Dis. 12:57. PubMed
  2. Dominguez Andres A, et al. 2020. bioRxiv. . PubMed
RRID
AB_2629594 (BioLegend Cat. No. 688202)

Antigen Details

Structure
The amino acid sequence of the Strep-tag is: Trp-Ser-His-Pro-Gln-Phe-Glu-Lys.
Function
Strep-tag can be attached to the N- or C-terminus of protein to become Strep-tag fused protein.
Biology Area
Cell Biology
Antigen References

1. Skerra A, et al. 2000. Methods Enzymol. 326:271.
2. Schmidt TG, et al. 2007. Nat. Protoc. 2:1528.

Gene ID
NA
UniProt
View information about Strep-Tag on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09.22.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
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