Purified anti-SPHK1 Antibody

Pricing & Availability
Clone
1A5SC (See other available formats)
Other Names
Sphingosine Kinase 1, SPK1, SK1
Isotype
Mouse IgG1
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Product Citations
publications
1A5SC_Purified_SPHK1_Antibody_WB_112613
Western blot analysis of extracts from Hela (lane 1) and PC-3 (lane 2) cells using anti-SPHK1 antibody (1A5SC). β-actin was used as loading control.
  • 1A5SC_Purified_SPHK1_Antibody_WB_112613
    Western blot analysis of extracts from Hela (lane 1) and PC-3 (lane 2) cells using anti-SPHK1 antibody (1A5SC). β-actin was used as loading control.
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658302 100 µg 212€
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Description

SPHK1 is a lipid kinase that catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P) which is a key sphingolipid signaling molecule involved in cell growth, survival, differentiation, and motility. As an important regulator of ceramide, sphingosine, and S1P, SPHK1 plays important roles in immunological, inflammatory diseases, and cancer. SPHK1 is overexpressed in various types of cancers, and upregulation of SPHK1 has been associated with tumor angiogenesis and resistance to radiation and chemotherapy. Agents targeting the SPHK1 have the potential as therapeutics for cancer, immunological, and inflammatory disorders.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SPHK1 peptide-KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2562878 (BioLegend Cat. No. 658302)

Antigen Details

Structure
384-470 amino acids, predicted molecular weight of 45-60 kD.
Distribution
Cytoplasm and nucleus.
Function
SPHK1 is a lipid kinase that catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate( S1P). It plays important roles in immunological, inflammatory diseases, and cancer.
Interaction
ACY1, calmodulin, and SPHKAP.
Ligand/Receptor
Sphingosine.
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Wallington-Beddoe CT, et al. 2013. Br. J. Haematol. 161:623.
2. Hisano Y, et al. 2012. J. Biochem. 152:305.
3. Carr JM, et al. 2013. Rev. Med. Virol. 23:73.
4. Maceyka M, et al. 2012. Trends Cell Biol. 22:50.
5. Shida D, et al. 2008. Curr. Drug Targets. 9:662.

Gene ID
8877 View all products for this Gene ID
UniProt
View information about SPHK1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11.26.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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