Purified anti-Siglec-11 Antibody

Pricing & Availability
Clone
4C4 (See other available formats)
Regulatory Status
RUO
Other Names
Sialic acid-binding Ig-like lectin 11, Sialic acid-binding lectin 11
Isotype
Mouse IgG2b
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Product Citations
publications
4C4_PURE_Siglec-11_Antibody_1_WB_120115
Protein (15 µg) harvested from MCF-7, Daudi and PBMCs were resolved by electrophoresis (A) or treated with 100U PNGase F (used to cleave N-linked glycans) before electrophoresis (B), transferred to nitrocellulose, and probed with purified monoclonal Siglec-11 (clone 4C4) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • 4C4_PURE_Siglec-11_Antibody_1_WB_120115
    Protein (15 µg) harvested from MCF-7, Daudi and PBMCs were resolved by electrophoresis (A) or treated with 100U PNGase F (used to cleave N-linked glycans) before electrophoresis (B), transferred to nitrocellulose, and probed with purified monoclonal Siglec-11 (clone 4C4) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • 4C4_PURE_Siglec-11_Antibody_2_WB_120115
    Protein (15 µg) harvested from MCF-7, Daudi and PBMCs were resolved by electrophoresis (A) or treated with 100U PNGase F (used to cleave N-linked glycans) before electrophoresis (B), transferred to nitrocellulose, and probed with purified monoclonal Siglec-11 (clone 4C4) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
681702 100 µg 180€
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Description

Siglec-11 is a 686 amino acid single-pass type I membrane protein that contains one Ig-like V-type domain and three Ig-like C2-type domains. In humans, Siglec-11 is widely expressed on tissue macrophages, which include microglia in the brain. No expression of Siglec-11 is seen in the brain compartment of non-human primates. Siglec-11 functions as an adhesion molecule that preferentially binds to α-2, 8-linked sialic acid trimers and mediates sialic acid binding to cells. It is a negative regulator of microglia, both related to inflammation and phagocytic function. In murine microglial cells with ectopic expression of human Siglec-11, stimulation of Siglec-11 could induce the transcription of the pro-inflammatory mediator genes, such as IL-1β and nitric oxide synthase-2. Phagocytosis of apoptotic neuronal material has shown to be reduced in Siglec-11 expressing microglia. Co-culture of Siglec-11 expressing microglia and neurons demonstrated neuroprotective functions of Siglec-11.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Siglec-11 fused to Fc region of human IgG.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Angata T, et al. 2002. J. Biol. Chem. 277:24466.
RRID
AB_2566496 (BioLegend Cat. No. 681702)

Antigen Details

Structure
698 amino acids, predicted molecular weight of 66 kD. Apparent molecular weight is 100-130 kD due to N-glycosylation.
Distribution

Plasma membrane.

Function
Adhesion molecule that mediates sialic-acid dependent binding to cells. The cytosolic domain of Siglec-11 is phosphorylated at tyrosine residue(s) upon pervanadate treatment of cells and then it recruits the protein-tyroisine phosphatases SHP-1 and SHP-2. Such interaction induces inhibitory cellular signals in immune response.
Interaction
Interacts with PTPN6/SHP-1 and PTPN11/SHIP-2 upon phosphorylation.
Ligand/Receptor
α-2,8-linked sialic acid trimers.
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, Siglec Molecules
Antigen References

1. Angata T, et al. 2002. J. Biol. Chem. 277:24466.
2. Hayakawa T, et al. 2005. Science 309:1693.
3. Kopatz J, et al. 2014. Glycobiology 9:794.
4. Wang Y, et al. 2010. J. Neurosci. 30:3482.
5. Linnartz B, et al. 2010. Int. J. Alzheimers Dis. 2010.

Gene ID
114132 View all products for this Gene ID
UniProt
View information about Siglec-11 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 08.06.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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