- 4C4 (See other available formats)
- Regulatory Status
- Other Names
- Sialic acid-binding Ig-like lectin 11, Sialic acid-binding lectin 11
- Mouse IgG2b
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- Product Citations
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Siglec-11 is a 686 amino acid single-pass type I membrane protein that contains one Ig-like V-type domain and three Ig-like C2-type domains. In humans, Siglec-11 is widely expressed on tissue macrophages, which include microglia in the brain. No expression of Siglec-11 is seen in the brain compartment of non-human primates. Siglec-11 functions as an adhesion molecule that preferentially binds to α-2, 8-linked sialic acid trimers and mediates sialic acid binding to cells. It is a negative regulator of microglia, both related to inflammation and phagocytic function. In murine microglial cells with ectopic expression of human Siglec-11, stimulation of Siglec-11 could induce the transcription of the pro-inflammatory mediator genes, such as IL-1β and nitric oxide synthase-2. Phagocytosis of apoptotic neuronal material has shown to be reduced in Siglec-11 expressing microglia. Co-culture of Siglec-11 expressing microglia and neurons demonstrated neuroprotective functions of Siglec-11.Product Details
- Antibody Type
- Host Species
- Recombinant Siglec-11 fused to Fc region of human IgG.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
IHC-P - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
(PubMed link indicates BioLegend citation)
- Angata T, et al. 2002. J. Biol. Chem. 277:24466.
AB_2566496 (BioLegend Cat. No. 681702)
- 698 amino acids, predicted molecular weight of 66 kD. Apparent molecular weight is 100-130 kD due to N-glycosylation.
- Adhesion molecule that mediates sialic-acid dependent binding to cells. The cytosolic domain of Siglec-11 is phosphorylated at tyrosine residue(s) upon pervanadate treatment of cells and then it recruits the protein-tyroisine phosphatases SHP-1 and SHP-2. Such interaction induces inhibitory cellular signals in immune response.
- Interacts with PTPN6/SHP-1 and PTPN11/SHIP-2 upon phosphorylation.
- α-2,8-linked sialic acid trimers.
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, Siglec Molecules
- Antigen References
1. Angata T, et al. 2002. J. Biol. Chem. 277:24466.
2. Hayakawa T, et al. 2005. Science 309:1693.
3. Kopatz J, et al. 2014. Glycobiology 9:794.
4. Wang Y, et al. 2010. J. Neurosci. 30:3482.
5. Linnartz B, et al. 2010. Int. J. Alzheimers Dis. 2010.
- Gene ID
- 114132 View all products for this Gene ID
- View information about Siglec-11 on UniProt.org
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